新分离的铜绿假单胞菌噬菌体vB_Pae_LC3I3的特性分析

Characterization of the newly isolated Pseudomonas phage vB_Pae_LC3I3.

作者信息

Xuan Guanhua, Kong Jiuna, Wang Yinfeng, Lin Hong, Wang Jingxue

机构信息

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.

Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China..

出版信息

Virus Res. 2023 Jan 2;323:198978. doi: 10.1016/j.virusres.2022.198978. Epub 2022 Oct 23.

DOI:10.1016/j.virusres.2022.198978

PMID:36288775

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10194125/

Abstract

Here, we report the genome sequence of a double-stranded DNA siphovirus, vB_Pae_LC3I3 infective for P. aeruginosa PA14. Phage vB_Pae_LC3I3 was identified as a linear double-stranded DNA phage of 49,926 bp with 59% G+C content. The vB_Pae_LC3I3 genome contains 78 open reading frames, and the function of 22 ORFs can be predicted. Genome analysis confirmed the lysogenic nature of this phage, which encodes the typical lysogen-related integrase and CI/Cro regulator. One-step growth curve revealed that the latent period of phage vB_Pae_LC3I3 lasted for 30 min. And vB_Pae_LC3I3 showed good temperature stability and pH stability. Based on electron microscopy, phylogenetic, and comparative genomic analyses, this novel Pseudomonas temperate phage represents a novel unassigned siphoviruses cluster. The study of phage vB_Pae_LC3I3 will provide basic information for further research on treatment of P. aeruginosa infections.

摘要

在此，我们报告了一种双链DNA长尾噬菌体病毒vB_Pae_LC3I3的基因组序列，该病毒可感染铜绿假单胞菌PA14。噬菌体vB_Pae_LC3I3被鉴定为一种线性双链DNA噬菌体，大小为49,926 bp，G+C含量为59%。vB_Pae_LC3I3基因组包含78个开放阅读框，其中22个ORF的功能可被预测。基因组分析证实了该噬菌体的溶原性质，其编码典型的与溶原相关的整合酶和CI/Cro调节因子。一步生长曲线显示噬菌体vB_Pae_LC3I3的潜伏期持续30分钟。并且vB_Pae_LC3I3表现出良好的温度稳定性和pH稳定性。基于电子显微镜、系统发育和比较基因组分析，这种新型假单胞菌温和噬菌体代表了一个未分类的新型长尾噬菌体病毒簇。对噬菌体vB_Pae_LC3I3的研究将为进一步研究铜绿假单胞菌感染的治疗提供基础信息。