Schulze Holthausen Johannes, Schregel Johannes, Sciascia Quentin L, Li Zeyang, Tuchscherer Armin, Vahjen Wilfried, Metges Cornelia C, Zentek Jürgen
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, 14195 Berlin, Germany.
Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology, 18196 Dummerstorf, Germany.
Microorganisms. 2022 Sep 25;10(10):1899. doi: 10.3390/microorganisms10101899.
Mortality, impaired development and metabolic dysfunctions of suckling low-birthweight piglets may be influenced by modulating the intestinal microbiome through glutamine supplementation. Therefore, this study examined whether glutamine supplementation may affect the colonic development and microbiome composition of male low- and normal-birthweight piglets at 5 and 12 days of age. Suckling piglets were supplemented orally with glutamine or alanine. Colonic digesta samples were obtained for 16S rDNA sequencing, determination of bacterial metabolites and histomorphological tissue analyses. Glutamine-supplemented piglets had lower concentrations of cadaverine and spermidine in the colonic digesta (p < 0.05) and a higher number of CD3+ colonic intraepithelial lymphocytes compared to alanine-supplemented piglets (p < 0.05). Low-birthweight piglets were characterised by a lower relative abundance of Firmicutes, the genera Negativibacillus and Faecalibacterium and a higher abundance of Alistipes (p < 0.05). Concentrations of cadaverine and total biogenic amines (p < 0.05) and CD3+ intraepithelial lymphocytes (p < 0.05) were lower in low- compared with normal-birthweight piglets. In comparison to the factor age, glutamine supplementation and birthweight were associated with minor changes in microbial and histological characteristics of the colon, indicating that ontogenetic factors play a more important role in intestinal development.
通过补充谷氨酰胺调节肠道微生物群，可能会影响哺乳期低出生体重仔猪的死亡率、发育受损和代谢功能障碍。因此，本研究探讨了补充谷氨酰胺是否会影响雄性低出生体重和正常出生体重仔猪在5日龄和12日龄时的结肠发育和微生物群组成。给哺乳仔猪口服补充谷氨酰胺或丙氨酸。采集结肠消化物样本进行16S rDNA测序、细菌代谢物测定和组织形态学分析。与补充丙氨酸的仔猪相比，补充谷氨酰胺的仔猪结肠消化物中尸胺和亚精胺浓度较低（p<0.05），CD3+结肠上皮内淋巴细胞数量较多（p<0.05）。低出生体重仔猪的特点是厚壁菌门、Negativibacillus属和粪杆菌属的相对丰度较低，而Alistipes属的丰度较高（p<0.05）。与正常出生体重仔猪相比，低出生体重仔猪的尸胺和总生物胺浓度（p<0.05）以及CD3+上皮内淋巴细胞数量（p<0.05）较低。与年龄因素相比，补充谷氨酰胺和出生体重与结肠微生物和组织学特征的微小变化有关，这表明个体发育因素在肠道发育中起更重要的作用。
