锐齿槲树叶提取物调节肉鸡肠道微生物群，提高抗氧化能力，缓解脂多糖诱导的炎症。

Acer truncatum leaves extract modulates gut microbiota, improves antioxidant capacity, and alleviates lipopolysaccharide-induced inflammation in broilers.

机构信息

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China; Key Laboratory of Livestock Biology, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China.

出版信息

Poult Sci. 2023 Oct;102(10):102951. doi: 10.1016/j.psj.2023.102951. Epub 2023 Jul 21.

DOI:10.1016/j.psj.2023.102951

PMID:37562124

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10432845/

Abstract

This study investigated the appropriate way of dietary Acer truncatum leaves (ATL) addition, the effect of disease prevention and its mechanism of action. In experiment 1, 192 Arbor Acres broilers were assigned to 4 treatment groups, fed with basal diets containing 2% bran, replacing it with primary and fermented ATL, and additional 0.3% ATL extract to the basal diet for 42 d, respectively. In experiment 2, 144 broilers were assigned to 3 treatment groups for 21-d trial: (1) C-N group, basal diets, and injected with 0.9% (w/v) sterile saline; (2) C-L group, basal diets, and injected with lipopolysaccharide (LPS); (3) T-L group, ATL diets and injected with LPS. In experiment 1, ATL significantly decreased the index of abdominal fat at 42 d (P < 0.05). ATL extract had a better ability to improve antioxidant capacity and reduce inflammatory levels among all treatment groups, which significantly decreased the content of MDA in the liver and ileum mucosa at 21 d, and increased the expression of IL-10 and Occludin in jejunal mucosa at 42 d (P < 0.05). In experiment 2, ATL significantly increased the level of T-AOC in the liver, decreased the expression of NF-κB in the jejunal mucosa and ileum mucosa (P < 0.05), and restored LPS-induced the changed level of CAT in jejunal mucosa, the expression of IL-6, Claudin-1, and ZO-1 in jejunal mucosa and IL-1β in ileum mucosa (P < 0.05). Analysis of gut microbiota indicated that ATL enhanced the abundances of Bacteroidota and reduced the proportion of Firmicutes (P < 0.05), and the changed levels of T-AOC in body, IL-1β, IL-6, IL-10, and NF-κB in jejunum mucosa and propionic acid in cecal were associated with gut microbiota. Collectively, our data showed that the extract of ATL had a better antioxidant and anti-inflammatory effects than primality and fermented. Extraction of ATL modulated intestinal microbiota, and had a protective effect on oxidative stress, inflammation, and intestinal barrier function in broilers challenged with LPS.

摘要

本研究旨在探讨添加食用 Acer truncatum 叶（ATL）的适宜方式、预防疾病的效果及其作用机制。在实验 1 中，将 192 只 Arbor Acres 肉鸡分为 4 个处理组，分别用基础日粮中添加 2%麸皮、替代初级和发酵 ATL、以及基础日粮中添加 0.3%ATL 提取物的方式饲养 42 天。在实验 2 中，将 144 只肉鸡分为 3 个处理组进行 21 天试验：（1）C-N 组，基础日粮，注射 0.9%（w/v）无菌生理盐水；（2）C-L 组，基础日粮，注射脂多糖（LPS）；（3）T-L 组，ATL 日粮，注射 LPS。在实验 1 中，ATL 在第 42 天显著降低了腹部脂肪指数（P<0.05）。ATL 提取物在所有处理组中具有更好的改善抗氧化能力和降低炎症水平的能力，在第 21 天显著降低了肝脏和回肠黏膜中 MDA 的含量，并在第 42 天增加了空肠黏膜中 IL-10 和 Occludin 的表达（P<0.05）。在实验 2 中，ATL 显著增加了肝脏中的 T-AOC 水平，降低了空肠和回肠黏膜中 NF-κB 的表达（P<0.05），并恢复了 LPS 诱导的空肠黏膜中 CAT 表达的变化水平、空肠黏膜中 IL-6、Claudin-1 和 ZO-1 的表达以及回肠黏膜中 IL-1β的表达（P<0.05）。肠道微生物群分析表明，ATL 增加了 Bacteroidota 的丰度，降低了 Firmicutes 的比例（P<0.05），并且机体中的 T-AOC 变化水平、空肠黏膜中的 IL-1β、IL-6、IL-10 和 NF-κB 以及盲肠中的丙酸与肠道微生物群有关。总的来说，我们的数据表明，ATL 的提取物比原始和发酵的提取物具有更好的抗氧化和抗炎作用。ATL 的提取物调节了肠道微生物群，并对 LPS 攻毒的肉鸡的氧化应激、炎症和肠道屏障功能具有保护作用。