Departamento de Microbiología, Universidad de Málaga, Málaga, Spain.
Área de Genética, Universidad de Málaga, Málaga, Spain.
PeerJ. 2022 Oct 24;10:e14248. doi: 10.7717/peerj.14248. eCollection 2022.
Pdp11 is a strain described as a probiotic for use in aquaculture. However, includes strains reported to be pathogenic or saprophytic to fish. Although the probiotic trait has been related to the presence of a group of genes in its genome, the existence of plasmids that could determine the probiotic or pathogenic character of this bacterium is unknown. In the present work, we searched for plasmids in several strains of that differ in their pathogenic and probiotic character. Under the different conditions tested, plasmids were only found in two of the five pathogenic strains, but not in the probiotic strain nor in the two saprophytic strains tested. Using a workflow integrating Sanger and Illumina reads, the complete consensus sequences of the plasmids were obtained. Plasmids differed in one ORF and encoded a putative replication initiator protein of the repB family, as well as proteins related to plasmid stability and a toxin-antitoxin system. Phylogenetic analysis showed some similarity to functional repB proteins of other species. The implication of these plasmids in the probiotic or pathogenic nature of is discussed.
Pdp11 是一种被描述为用于水产养殖的益生菌。然而，它包括被报道对鱼类具有致病性或腐生性的菌株。尽管益生菌特性与基因组中一组基因的存在有关，但该细菌的益生菌或致病性特征是否由质粒决定尚不清楚。在本工作中，我们在不同致病性和益生菌特性的几种 Pdp11 菌株中寻找质粒。在测试的不同条件下，仅在两个致病性菌株中发现了质粒，但在益生菌菌株和两个测试的腐生菌株中没有发现。使用整合 Sanger 和 Illumina 读数的工作流程，获得了质粒的完整一致序列。质粒在一个 ORF 上有所不同，编码 repB 家族的假定复制起始蛋白，以及与质粒稳定性和毒素-抗毒素系统相关的蛋白质。系统发育分析显示与其他 Pdp11 物种的功能 repB 蛋白有一定的相似性。本文讨论了这些质粒在 Pdp11 益生菌或致病性中的作用。
