Ahmad Shujaat, Khan Idrees, Saeed Khalid, Ahmad Hanif, Alam Aftab, Almehmadi Mazen, Alsaiari Ahad Amer, Haitao Yu, Ahmad Manzoor
Department of Chemistry, University of Malakand, Charsadda, Khyber Pakhtunkhwa, Paksitan.
College of Chemistry and Materials Science, Hebei Normal University, Shijiazhuang, China.
Front Pharmacol. 2022 Oct 7;13:1008182. doi: 10.3389/fphar.2022.1008182. eCollection 2022.
The conventional chemical methods of nanoparticles synthesis have been effectively replaced by nanoparticle synthesis mediated by plants. The current study describes the environmental friendly synthesis of chromium oxide nanoparticles (CrO NPs) using plant extract. The synthesis of CrO NPs was validated by UV/VIS spectroscopy, Energy Dispersive X-Ray (EDX), Scanning Electron Microscopy (SEM), and X-ray diffraction (XRD) studies. The appearance of the Sharpe peak at 460 nm in the UV/Vis spectrum and the colour change caused by surface plasma resonance confirmed the formation of CrO NPs. The EDX spectrum of CrO nanoparticles revealed the presence of carbon, oxygen, and chromium, while SEM analysis revealed an irregular round morphology (with a size below 400 nm). In addition, XRD studies suggested their crystalline nature by the characteristic peaks at 34° and 36° and 42° (2Ɵ), respectively. The green synthesized CrO NPs showed promise as cholinesterase inhibitor at tested concentrations (62.5-1,000 μg/ml), with IC values of 120 and 100 μg/ml against Acetylcholinesterase (AChE) and Butyrylcholinesterase (BChE), respectively. The results suggested that the green synthesized CrO NPs could be used in the future to stop enzyme from working and for other biological activities.
传统的纳米颗粒合成化学方法已被植物介导的纳米颗粒合成有效取代。当前研究描述了使用植物提取物对氧化铬纳米颗粒（CrO NPs）进行环境友好型合成。通过紫外/可见光谱、能量色散X射线（EDX）、扫描电子显微镜（SEM）和X射线衍射（XRD）研究对CrO NPs的合成进行了验证。紫外/可见光谱中460 nm处尖锐峰的出现以及表面等离子体共振引起的颜色变化证实了CrO NPs的形成。CrO纳米颗粒的EDX光谱显示存在碳、氧和铬，而SEM分析显示其形态为不规则圆形（尺寸小于400 nm）。此外，XRD研究通过分别在34°、36°和42°（2Ɵ）处的特征峰表明了它们的晶体性质。绿色合成的CrO NPs在测试浓度（62.5 - 1000 μg/ml）下显示出作为胆碱酯酶抑制剂的潜力，对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的IC值分别为120和100 μg/ml。结果表明，绿色合成的CrO NPs未来可用于使酶失活以及其他生物活性。
