Kwon Hee Jung, Oh Joo Yeon, Lee Kwang Seon, Lim Hyun Kyung, Lee Jisun, Yoon Hye-Ran, Jung Joohee
Department of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
Duksung Innovative Drug Center, Duksung Women's University, Seoul 01369, Republic of Korea.
Int J Anal Chem. 2022 Oct 27;2022:6007158. doi: 10.1155/2022/6007158. eCollection 2022.
Liver cancer metastasis is known to be a poor prognosis and a leading cause of mortality. To overcome low therapeutic efficacy, understanding the physiological properties of liver cancer metastasis is required. However, the metastatic lesion is heterogeneous and complex. We investigate the distribution of lipids using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) in an experimental metastasis model. We obtained the differentially expressed mass peaks in comparison between normal sites and metastatic lesions. The relationship of mass to charge ratio (m/z) and intensity were measured, m/z-indicated species were analyzed by MALDI-MS/MS analysis, and identification of these mass species was confirmed using the METASPACEannotation platform and Lipid Maps®. MALDI-MSI at m/z 725.6, 734.6, 735.6, 741.6, 742.6, 744.6, 756.6, and 772.6 showed significantly higher intensity, consistent with the metastatic lesions in hematoxylin-stained tissues. Sphingomyelin SM [d18:0/16:1], phosphatidylcholine (PC) [32:0], PC [31:0], PC [31:1], and PE [36:2] were highly expressed in metastatic lesions. Our results could provide information for understanding metastatic lesions. It suggests that the found lipids could be a biomarker for the diagnosis of metastatic lesions.
肝癌转移预后较差，是导致死亡的主要原因。为了克服治疗效果不佳的问题，需要了解肝癌转移的生理特性。然而，转移病灶具有异质性和复杂性。我们在实验性转移模型中使用基质辅助激光解吸/电离质谱成像（MALDI-MSI）研究脂质分布。我们获得了正常部位和转移病灶之间差异表达的质谱峰。测量了质荷比（m/z）与强度的关系，通过MALDI-MS/MS分析对m/z指示的物种进行了分析，并使用METASPACE注释平台和Lipid Maps®确认了这些质谱物种的鉴定。在m/z 725.6、734.6、735.6、741.6、742.6、744.6、756.6和772.6处的MALDI-MSI显示强度显著更高，与苏木精染色组织中的转移病灶一致。鞘磷脂SM [d18:0/16:1]、磷脂酰胆碱（PC）[32:0]、PC [31:0]、PC [31:1]和PE [36:2]在转移病灶中高表达。我们的结果可为理解转移病灶提供信息。这表明所发现的脂质可能是转移病灶诊断中的生物标志物。
