Cao Yanan, Zhang Shuoshuo, Huang Yanjie, Zhang Shuai, Wang Haifei, Bao Wenbin
Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-product Safety, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2022 Feb 13;12(4):458. doi: 10.3390/ani12040458.
Porcine epidemic diarrhea (PED), one of the serious enteric diseases caused by the porcine epidemic diarrhea virus (PEDV), is responsible for enormous economic losses in the global swine industry. However, available commercial vaccines fail to protect pigs from PEDV infection due to the appearance of PEDV variants. Hence, it is necessary to find an effective and cost-efficient natural product to protect pigs from PEDV infection. In this study, we first found that an aqueous leaf extract of (MOE) exhibited antiviral activity in response to PEDV infection. Furthermore, time-of-addition experiments revealed that MOE inhibited PEDV replication rather than attachment and internalization. Mechanistically, MOE significantly suppressed the production of reactive oxygen species (ROS) and malondialdehyde (MDA) induced by PEDV infection, and restored glutathione peroxidase (GSH-Px) activity. Importantly, the addition of MOE alleviated oxidative stress and the expression of inflammatory cytokines and resulted in fewer apoptotic cells during PEDV infection. These results indicated that MOE might be an effective anti-PEDV drug used to control PED disease and may be helpful in developing a new prophylactic and therapeutic strategy against PEDV.
猪流行性腹泻（PED）是由猪流行性腹泻病毒（PEDV）引起的严重肠道疾病之一，给全球养猪业造成了巨大的经济损失。然而，由于PEDV变异株的出现，现有的商业疫苗无法保护猪免受PEDV感染。因此，有必要找到一种有效且经济高效的天然产物来保护猪免受PEDV感染。在本研究中，我们首先发现（MOE）的叶水提取物对PEDV感染具有抗病毒活性。此外，添加时间实验表明，MOE抑制PEDV复制而非附着和内化。机制上，MOE显著抑制PEDV感染诱导的活性氧（ROS）和丙二醛（MDA）的产生，并恢复谷胱甘肽过氧化物酶（GSH-Px）活性。重要的是，添加MOE减轻了氧化应激和炎性细胞因子的表达，并减少了PEDV感染期间的凋亡细胞。这些结果表明，MOE可能是一种用于控制PED疾病的有效抗PEDV药物，可能有助于开发针对PEDV的新的预防和治疗策略。
