Küçükkurt İsmail, Akbel Erten, İnce Sinan, Acaröz Damla Arslan, Demirel Hasan Hüseyin, Kan Fahriye
Department of Biochemistry, Faculty of Veterinary Medicine, 03200 Afyon Kocatepe University, Afyonkarahisar, Turkey.
Usak Health Training School, Usak University, 64100 Usak, Turkey.
Toxicol Res (Camb). 2022 Sep 1;11(5):812-818. doi: 10.1093/toxres/tfac059. eCollection 2022 Oct.
Cyclophosphamide (CP)-also known as cytophosphan-is an alkylating agent that has many side effects in humans and rats. Rats were divided into 5 different groups to evaluate the protective effect of escin (ES) obtained from the horse-chestnut plant () against acute damage induce by CP. Groups: control group, ethanol group, ES group (100 mg/kg body weight (bw) ES for 14 days by gastric gavage), ES + CP group (100 mg/kg bw ES for 14 days by gastric gavage and 75 mg/kg bw CP i.p. on 14th day), and CP group (75 mg/kg bw CP i.p. on 14th day). After the experiment was completed, blood and tissue samples (liver, kidney, heart, brain, lung, and testis) were taken from the rats under anesthesia. When the CP group was compared with the control group, an increase was observed in the level of Malondialdehyde (MDA) in blood and all tissues except the lung, but when it was given together with escin, there was a decrease except kidney and lung ( < 0.05). Glutathione (GSH) level decreased in the blood and all tissues when CP was given, whereas an increase was observed in the heart, brain, and lung when given with escin ( < 0.05). There was no statistical change in the activities of superoxide dismutase and catalase enzymes in all tissues. ES reduced CP-induced damage in all tissues except the kidney. As a result, it was determined that ES had a protective effect against CP-induced tissue damage in rats due to its antioxidant properties.
环磷酰胺（CP）——也被称为癌得星——是一种烷化剂，在人类和大鼠中会产生多种副作用。将大鼠分为5个不同组，以评估从七叶树植物中提取的七叶皂苷（ES）对CP诱导的急性损伤的保护作用。分组：对照组、乙醇组、ES组（通过胃管灌胃给予100毫克/千克体重（bw）的ES，持续14天）、ES + CP组（通过胃管灌胃给予100毫克/千克bw的ES，持续14天，并在第14天腹腔注射75毫克/千克bw的CP）以及CP组（在第14天腹腔注射75毫克/千克bw的CP）。实验完成后，在麻醉状态下从大鼠身上采集血液和组织样本（肝脏、肾脏、心脏、大脑、肺和睾丸）。当将CP组与对照组进行比较时，发现血液以及除肺以外的所有组织中的丙二醛（MDA）水平均有所升高，但当与七叶皂苷一起给药时，除肾脏和肺外，MDA水平有所下降（P < 0.05）。给予CP时，血液和所有组织中的谷胱甘肽（GSH）水平均下降，而与七叶皂苷一起给药时，心脏、大脑和肺中的GSH水平有所升高（P < 0.05）。所有组织中超氧化物歧化酶和过氧化氢酶的活性均无统计学变化。ES减轻了除肾脏以外所有组织中CP诱导的损伤。结果表明，ES因其抗氧化特性对CP诱导的大鼠组织损伤具有保护作用。
