Oguma Yo, Kuroda Yasumasa, Wakao Shohei, Kushida Yoshihiro, Dezawa Mari
Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, 2-1 Seiryomachi, Aobaku, Sendai 980-8575, Japan.
iScience. 2022 Oct 20;25(11):105395. doi: 10.1016/j.isci.2022.105395. eCollection 2022 Nov 18.
Somatic stem cells are advantageous research targets for understanding the properties required to maintain stemness. Human bone marrow-mesenchymal stromal cells (BM-MSCs) were separated into pluripotent-like SSEA-3(+) Muse cells (Muse-MSCs) and multipotent SSEA-3(-) MSCs (MSCs) and were subjected to single-cell RNA sequencing analysis. Compared with MSCs, Muse-MSCs exhibited higher expression levels of the p53 repressor ; signal acceptance-related genes EGF, VEGF, PDGF, WNT, TGFB, INHB, and CSF; ribosomal protein; and glycolysis and oxidative phosphorylation. Conversely, MSCs had higher expression levels of FGF and ANGPT; Rho family and caveola-related genes; amino acid and cofactor metabolism; MHC class I/II, and lysosomal enzyme genes than Muse-MSCs. Unsupervised clustering further divided Muse-MSCs into two clusters stratified by the expression of cell cycle-related genes, and MSCs into three clusters stratified by the expression of cell cycle-, cytoskeleton-, and extracellular matrix-related genes. This study evaluating the differentiation ability of BM-MSC subpopulations provides intriguing insights for understanding stemness.
体细胞干细胞是理解维持干性所需特性的有利研究靶点。人骨髓间充质基质细胞（BM-MSCs）被分离为多能样SSEA-3(+)缪斯细胞（Muse-MSCs）和多能SSEA-3(-) MSCs（MSCs），并进行单细胞RNA测序分析。与MSCs相比，Muse-MSCs表现出更高水平的p53阻遏物表达；信号接受相关基因EGF、VEGF、PDGF、WNT、TGFB、INHB和CSF；核糖体蛋白；以及糖酵解和氧化磷酸化。相反，MSCs在FGF和ANGPT；Rho家族和小窝相关基因；氨基酸和辅因子代谢；MHC I/II类以及溶酶体酶基因方面的表达水平高于Muse-MSCs。无监督聚类进一步将Muse-MSCs分为两个由细胞周期相关基因表达分层的簇，将MSCs分为三个由细胞周期、细胞骨架和细胞外基质相关基因表达分层的簇。这项评估BM-MSC亚群分化能力的研究为理解干性提供了有趣的见解。
