Xu Dan, Wang Xiaojuan, Wang Weimin, Zhang Deyin, Li Xiaolong, Zhang Yukun, Zhao Yuan, Cheng Jiangbo, Zhao Liming, Wang Jianghui, Lin Changchun, Yang Xiaobin, Weng Xiuxiu, Zhang Xiaoxue, Zheng Wenxin
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Anim Biotechnol. 2023 Dec;34(9):4600-4607. doi: 10.1080/10495398.2023.2174877. Epub 2023 Feb 13.
In this study, a single nucleotide polymorphism of (hydroxytryptamine receptor 4) was detected using DNA sequencing and KASPar (Kompetitive Allele-Specific PCR) technique with the aim of analyzing its effect on growth traits in 1102 sheep. A synonymous mutation g.101220 C > T located on the fifth intron of the ovis gene was detected, and association analysis showed that this mutation was significantly associated with growth traits in sheep ( ＜.05), with TT genotypes having significantly lower body weight, height, length and chest circumference than TC and CC genotypes. It showed that the polymorphism of this locus was significantly associated with growth traits in sheep. In addition, qRT-PCR results showed that was expressed in different tissues of sheep. It is highly expressed in the liver, spleen and duodenum. As important metabolic, immune and digestive absorption organs in animals, the above tissues can regulate the excitability of intestinal smooth muscle by participating in the body metabolism and nutrient metabolism of sheep, so that sheep can show better growth characteristics. In conclusion, the polymorphic locus identified in gene can be used as candidate molecular marker in sheep breeding.
在本研究中，采用DNA测序和KASPar（竞争性等位基因特异性PCR）技术检测了羟色胺受体4的单核苷酸多态性，旨在分析其对1102只绵羊生长性状的影响。检测到位于绵羊基因第五内含子上的同义突变g.101220 C＞T，关联分析表明该突变与绵羊生长性状显著相关（P＜0.05），TT基因型的体重、体高、体长和胸围显著低于TC和CC基因型。结果表明，该位点的多态性与绵羊生长性状显著相关。此外，qRT-PCR结果显示该基因在绵羊不同组织中均有表达，在肝脏、脾脏和十二指肠中高表达。作为动物重要的代谢、免疫和消化吸收器官，上述组织可通过参与绵羊的机体代谢和营养代谢来调节肠道平滑肌的兴奋性，从而使绵羊表现出更好的生长特性。综上所述，在绵羊基因中鉴定出的多态性位点可作为绵羊育种的候选分子标记。
