College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Institute of Animal Husbandry Quality Standards, Xinjiang Academy of Animal Sciences, Urumqi, Xinjiang, China.
Anim Biotechnol. 2024 Nov;35(1):2295926. doi: 10.1080/10495398.2023.2295926. Epub 2023 Dec 27.
The body size traits are major traits in livestock, which intuitively displays the development of the animal's bones and muscles. This study used PCR amplification, Sanger sequencing, KASPar genotyping, and quantitative real-time reverse transcription PCR (qRT-PCR) to analyze the Single-nucleotide polymorphism and expression characteristics of Argonaute RISC catalytic component 2 () and Plectin () genes in Hu sheep. Two intron mutations were found in Hu sheep, which were g.51700 A > C and g.23157 C > T, respectively. Through association analysis of two mutation sites and body size traits, it was found that g.51700 A > C mainly affects the chest and cannon circumference of Hu sheep of while g.23157 C mainly affects body height and body length. The combined genotypes of and genes with body size traits showed SNPs at the g.51700 A > C and g.23157 C > T loci significantly improved the body size traits of Hu sheep. In addition, the gene has the highest expression levels in the heart, rumen, and tail fat, and the gene is highly expressed in the heart. These two loci can provide new research ideas for improving the body size traits of Hu sheep.
体尺性状是家畜的主要性状，直观地显示了动物骨骼和肌肉的发育情况。本研究采用 PCR 扩增、Sanger 测序、KASPar 基因分型和实时荧光定量 RT-PCR(qRT-PCR)技术，分析了湖羊 Argonaute RISC 催化成分 2()和 Plectin()基因的单核苷酸多态性和表达特征。在湖羊中发现了两个内含子突变，分别为 g.51700 A>G 和 g.23157 C>T。通过对两个突变位点与体尺性状的关联分析发现，g.51700 A>G 主要影响湖羊的胸宽和管围，而 g.23157 C>T 主要影响体高和体长。与体尺性状相关的和基因的组合基因型在 g.51700 A>G 和 g.23157 C>T 位点表现出 SNP，显著改善了湖羊的体尺性状。此外，基因在心脏、瘤胃和尾脂中表达水平最高，基因在心脏中高度表达。这两个位点为提高湖羊体尺性状提供了新的研究思路。
