Periodontology and Periodontal Medicine, Centre for Host-Microbiome Interactions, Faculty of Dentistry, Oral and Craniofacial Sciences, Kings College London, Guy's and St Thomas' NHS Foundation Trust, London SE1 9RT, UK.
Microbial Diseases, Eastman Dental Institute, University College London, London WC1E 6BT, UK.
Int J Environ Res Public Health. 2022 Oct 28;19(21):14088. doi: 10.3390/ijerph192114088.
Peri-implantitis is a disease influenced by dysbiotic microbial communities that play a role in the short- and long-term outcomes of its clinical treatment. The ecological triggers that establish the progression from peri-implant mucositis to peri-implantitis remain unknown. This investigation describes the development of a novel in vitro microcosm biofilm model. Biofilms were grown over 30 days over machined titanium discs in a constant depth film fermentor (CDFF), which was inoculated (I) with pooled human saliva. Following longitudinal biofilm sampling across peri-implant health (PH), peri-implant mucositis (PM), and peri-implantitis (PI) conditions, the characterisation of the biofilms was performed. The biofilm analyses included imaging by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), selective and non-selective culture media of viable biofilms, and 16S rRNA gene amplification and sequencing. Bacterial qualitative shifts were observed by CLSM and SEM across conditions, which were defined by characteristic phenotypes. A total of 9 phyla, 83 genera, and 156 species were identified throughout the experiment. The phyla , , , , and showed the highest prevalence in PI conditions. This novel in vitro microcosm model provides a high-throughput alternative for growing microcosm biofilms resembling an in vitro progression from PH-PM-PI conditions.
种植体周围炎是一种受生态失调微生物群落影响的疾病，这些微生物群落对其临床治疗的短期和长期结果起着作用。导致种植体周围黏膜炎向种植体周围炎进展的生态触发因素尚不清楚。本研究描述了一种新型体外微宇宙生物膜模型的开发。在恒深液流发酵器（CDFF）中，将经过机械加工的钛盘上的生物膜培养 30 天，并用混合的人唾液接种（I）。在种植体周围健康（PH）、种植体周围黏膜炎（PM）和种植体周围炎（PI）条件下进行纵向生物膜采样后，对生物膜进行了特征描述。生物膜分析包括共聚焦激光扫描显微镜（CLSM）和扫描电子显微镜（SEM）成像、可培养生物膜的选择性和非选择性培养基以及 16S rRNA 基因扩增和测序。通过 CLSM 和 SEM 观察到整个实验过程中条件下的细菌定性变化，这些变化由特征表型定义。共鉴定出 9 个门、83 个属和 156 个种。在 PI 条件下，、、、、和 出现的频率最高。这种新型体外微宇宙模型提供了一种高通量的替代方法，可以生长类似于 PH-PM-PI 条件下体外进展的微宇宙生物膜。
