Department of Physiology, Faculty of Medicine, Suez Canal University, Ismailia 8366004, Egypt.
Department of Drug and Health Science, University of Catania, 95125 Catania, Italy.
Molecules. 2022 Oct 26;27(21):7259. doi: 10.3390/molecules27217259.
Silver nanoparticles (AgNPs) are widely used commercially due to their antimicrobial effects. Little is known about the effect of AgNPs on neural transmission and pain response. The aim of this study was to assess the anti-nociceptive activity of AgNPs. AgNPs were prepared at 16 ug/mL, white albino rats were injected with various doses of AgNPs, and challenged using a hot-plate test and paw withdrawal latency (PWL) was measured. The chronic constriction injury (CCI) model was utilized to evaluate the pedal withdrawal reflex and tail withdrawal reflex. An electrophysiological study was conducted utilizing colon longitudinal muscle strips. AgNPs increased the latency of PWL in a dose-dependent matter over the duration of 6 h. The paw withdrawal threshold in animals with CCI significantly increased after AgNPs administration. In isolated colon longitudinal muscle strips, AgNPs significantly reduced the colonic migrating motor complexes (MMCs) and contraction. This action was completely reversed after removing the AgNPs and adding acetylcholine to the preparation. In this study, AgNPs showed significant anti-nociception properties. To our knowledge, this is the first report to describe this pharmacological action of AgNPs.
银纳米粒子（AgNPs）由于其抗菌作用而被广泛应用于商业领域。然而，人们对 AgNPs 对神经传递和疼痛反应的影响知之甚少。本研究旨在评估 AgNPs 的抗伤害活性。AgNPs 以 16ug/ml 的浓度制备，将不同剂量的 AgNPs 注射到白色白化大鼠体内，利用热板试验和足底回缩潜伏期（PWL）进行检测。慢性缩窄性损伤（CCI）模型用于评估足部回缩反射和尾部回缩反射。利用结肠纵向肌条进行电生理研究。AgNPs 在 6 小时的时间内以剂量依赖的方式增加 PWL 的潜伏期。CCI 动物在给予 AgNPs 后，其足部回缩阈值显著增加。在分离的结肠纵向肌条中，AgNPs 显著减少了结肠移行性运动复合波（MMCs）和收缩。将 AgNPs 从制剂中去除并添加乙酰胆碱后，这种作用完全逆转。在本研究中，AgNPs 表现出显著的镇痛作用。据我们所知，这是首次描述 AgNPs 的这种药理作用。
