Department of Natural Products Chemistry, Medical University of Lublin, 20-093 Lublin, Poland.
Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation-State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland.
Molecules. 2022 Nov 3;27(21):7529. doi: 10.3390/molecules27217529.
(Family Asteraceae) is widely distributed in the Eastern desert of Egypt. It is a promising plant material for phytochemical analysis and pharmacologic studies, and so far, its specific metabolites and biological activity have not yet been thoroughly investigated. Herein, we report on the detailed phytochemical study using UPLC-Q-TOF-MS approach. This analysis allowed the putative annotation of 48 metabolites belonging to various phytochemical classes, including mostly sesquiterpenes, flavonoids, and phenolic acids. Further, zebrafish embryotoxicity has been carried out, where 100 µg/mL extract incubated for 72 h resulted in a slow touch response of the 10 examined larvae, which might be taken as a sign of a disturbed peripheral nervous system. Results of in vitro testing indicate moderate cytotoxicity towards VERO, FaDu, and HeLa cells with CC values between 91.6 and 101.7 µg/mL. However, selective antineoplastic activity in RKO cells with CC of 54.5 µg/mL was observed. To the best of our knowledge, this is the first comprehensive profile of secondary metabolites that provides chemical-based evidence for its biological effects. A further investigation should be carried out to precisely define the underlying mechanisms of toxicity.
（菊科）广泛分布于埃及东部沙漠。它是一种很有前途的植物材料，可用于植物化学分析和药理学研究，迄今为止，其特定代谢物和生物活性尚未得到彻底研究。在此，我们报告了使用 UPLC-Q-TOF-MS 方法进行的详细植物化学研究。该分析能够对 48 种属于各种植物化学类别的代谢产物进行假设注释，包括大多数倍半萜、类黄酮和酚酸。此外，还进行了斑马鱼胚胎毒性试验，在孵育 72 小时后，100µg/mL 的提取物导致 10 个被检测的幼虫出现缓慢触摸反应，这可能被视为外周神经系统受损的迹象。体外测试结果表明，对 VERO、FaDu 和 HeLa 细胞具有中等细胞毒性，CC 值在 91.6 至 101.7µg/mL 之间。然而，在 RKO 细胞中观察到选择性抗肿瘤活性，CC 值为 54.5µg/mL。据我们所知，这是首次对其次生代谢物进行全面分析，为其生物效应提供了基于化学的证据。应进一步进行研究以精确定义毒性的潜在机制。
