Macchione Micaela A, Bedoya Dariana Aristizábal, Rivero-Buceta Eva, Botella Pablo, Strumia Miriam C
Centro de Investigaciones y Transferencia de Villa María (CIT Villa María), CONICET-UNVM, Arturo Jauretche 1555, Villa María, Córdoba X5900LQC, Argentina.
Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Av. Haya de la Torre esq. Av. Medina Allende, Córdoba X5000HUA, Argentina.
Nanomaterials (Basel). 2022 Oct 30;12(21):3835. doi: 10.3390/nano12213835.
Polymeric-inorganic hybrid nanomaterials have emerged as novel multifunctional platforms because they combine the intrinsic characteristics of both materials with unexpected properties that arise from synergistic effects. In this work, hybrid nanogels based on mesoporous silica nanoparticles, oligo (ethylene glycol) methacrylates, and acidic moieties were developed employing ultrasound-assisted free radical precipitation/dispersion polymerization. Chemical structure was characterized by infrared spectroscopy and nuclear magnetic resonance. Hydrodynamic diameters at different temperatures were determined by dynamic light scattering, and cloud point temperatures were determined by turbidimetry. Cell viability in fibroblast (NIH 3T3) and human prostate cancer (LNCaP) cell lines were studied by a standard colorimetric assay. The synthetic approach allows covalent bonding between the organic and inorganic components. The composition of the polymeric structure of hybrid nanogels was optimized to incorporate high percentages of acidic co-monomer, maintaining homogeneous nanosized distribution, achieving appropriate volume phase transition temperature values for biomedical applications, and remarkable pH response. The cytotoxicity assays show that cell viability was above 80% even at the highest nanogel concentration. Finally, we demonstrated the successful cell inhibition when they were treated with camptothecin-loaded hybrid nanogels.
聚合物-无机杂化纳米材料已成为新型多功能平台，因为它们将两种材料的固有特性与协同效应产生的意外性能结合在一起。在这项工作中，采用超声辅助自由基沉淀/分散聚合法制备了基于介孔二氧化硅纳米颗粒、聚乙二醇甲基丙烯酸酯和酸性基团的杂化纳米凝胶。通过红外光谱和核磁共振对化学结构进行了表征。通过动态光散射测定不同温度下的流体动力学直径，通过比浊法测定浊点温度。通过标准比色法研究了成纤维细胞（NIH 3T3）和人前列腺癌（LNCaP）细胞系中的细胞活力。该合成方法允许有机和无机成分之间形成共价键。对杂化纳米凝胶聚合物结构的组成进行了优化，以纳入高百分比的酸性共聚单体，保持均匀的纳米尺寸分布，获得适合生物医学应用的体积相变温度值，并具有显著的pH响应。细胞毒性试验表明，即使在最高纳米凝胶浓度下，细胞活力仍高于80%。最后，我们证明了用负载喜树碱的杂化纳米凝胶处理细胞时成功实现了细胞抑制。
