关于新出现的广泛耐药和多重耐药菌的分子分型、毒力特征以及表型和基因型耐药模式的首次报告 。 你提供的原文似乎不完整，“in.”后面缺少具体内容。
A First Report of Molecular Typing, Virulence Traits, and Phenotypic and Genotypic Resistance Patterns of Newly Emerging XDR and MDR in .
作者信息
Algammal Abdelazeem M, Ibrahim Reham A, Alfifi Khyreyah J, Ghabban Hanaa, Alghamdi Saad, Kabrah Ahmed, Khafagy Ahmed R, Abou-Elela Gehan M, Abu-Elala Nermeen M, Donadu Matthew Gavino, El-Tarabili Reham M
机构信息
Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
National Institute of Oceanography and Fisheries, Cairo 11516, Egypt.
出版信息
Pathogens. 2022 Oct 29;11(11):1262. doi: 10.3390/pathogens11111262.
is associated with substantial economic losses in the fish industry and with food-borne illness in humans. This study aimed to determine the prevalence, antibiogram profiles, sequence analysis, virulence and antimicrobial resistance genes, and pathogenicity of recovered from . A total of 80 fish were randomly gathered from various private farms in Suez Province, Egypt. Subsequently, samples were subjected to clinical, post-mortem, and bacteriological examinations. The retrieved isolates were tested for sequence analysis, antibiogram profile, pathogenicity, and PCR detection of virulence and resistance genes. The prevalence of in the examined was 22.5 % (18/80). The phylogenetic analyses revealed that the tested strains shared high genetic similarity with other strains from India, UK, and China. Using PCR it was revealed that the retrieved isolates harbored the A, , , AII, , , and virulence genes with prevalence of 100%, 82.9%, 61.7%, 55.3%, 44.7%, 36.17%, and 29.8%, respectively. Our findings revealed that 29.8% (14/47) of the retrieved strains were XDR to nine antimicrobial classes and carried , , A, A1, and 1 resistance genes. Likewise, 19.1% (9/47) of the obtained strains were MDR to eight classes and possessed , , A, A1, and 1 genes. The pathogenicity testing indicated that the mortality rates positively correlated with the prevalence of virulence-determinant genes. To our knowledge, this is the first report to reveal the occurrence of XDR and MDR in , an emergence that represents a risk to public health. Emerging XDR and MDR in frequently harbored A, , , AII, and virulence genes, and , 1, A, , , and A1 resistance genes.
与渔业的重大经济损失以及人类食源性疾病相关。本研究旨在确定从……分离出的……的流行率、抗菌谱、序列分析、毒力和抗菌抗性基因以及致病性。总共从埃及苏伊士省的各个私人养殖场随机采集了80条鱼。随后，对样本进行临床、尸检和细菌学检查。对分离得到的菌株进行序列分析、抗菌谱、致病性以及毒力和抗性基因的PCR检测。在所检测的……中，……的流行率为22.5%（18/80）。系统发育分析表明，所检测的……菌株与来自印度、英国和中国的其他……菌株具有高度的遗传相似性。通过PCR发现，分离得到的……菌株携带A、……、……、AII、……、……和……毒力基因，其流行率分别为100%、82.9%、61.7%、55.3%、44.7%、36.17%和29.8%。我们的研究结果表明，所分离的……菌株中有29.8%（14/47）对九种抗菌类别呈现广泛耐药，并携带……、……、A、A1和……抗性基因。同样，所获得的……菌株中有19.1%（9/47）对八种类别呈现多重耐药，并拥有……、……、A、A1和……基因。致病性测试表明死亡率与毒力决定基因的流行率呈正相关。据我们所知，这是首次报告揭示……中广泛耐药和多重耐药的……的出现，这一情况对公共卫生构成风险。……中新兴的广泛耐药和多重耐药……通常携带A、……、……、AII和……毒力基因，以及……、……、1、A、……、……和A1抗性基因。
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