Lark C A, Riazi J, Lark K G
J Bacteriol. 1978 Dec;136(3):1008-17. doi: 10.1128/jb.136.3.1008-1017.1978.
Normally, bacteria cease DNA replication in the absence of protein synthesis. A variety of treatments, such as thymine starvation or a shift-up to rich medium, lead to continued DNA replication in the absence of protein synthesis. Mutants are described which always terminate replication under these conditions. These conditional lethal mutants, dnaT1 and dnaT2, contransduce with serB and dnaC. The mutation also affects cell division. All aspects of the mutant phenotype (obligatory termination of replication, temperature sensitivity of DNA replication and growth, and aberrant cell division at permissive growth temperatures) were transdominant to the wild-type phenotype. Episomes carrying the dnaT mutation appeared to be unstable. The existence of such a dominant mutation was predicted by a model of chromosome termination proposed by Kogoma and Lark (J. Mol. Biol. 94:243-256, 1975).
正常情况下，细菌在缺乏蛋白质合成时会停止DNA复制。多种处理方式，如胸腺嘧啶饥饿或转换至丰富培养基，会导致在缺乏蛋白质合成的情况下DNA继续复制。已描述了在这些条件下总是终止复制的突变体。这些条件致死突变体dnaT1和dnaT2与serB和dnaC共转导。该突变也影响细胞分裂。突变体表型的所有方面（复制的强制性终止、DNA复制和生长的温度敏感性以及在允许生长温度下的异常细胞分裂）相对于野生型表型都是显性的。携带dnaT突变的附加体似乎不稳定。Kogoma和Lark（《分子生物学杂志》94:243 - 256, 1975）提出的染色体终止模型预测了这种显性突变的存在。
