The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR4 7UH, Norwich, UK.
The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, NR4 7UH, Norwich, UK.
Curr Opin Plant Biol. 2022 Dec;70:102311. doi: 10.1016/j.pbi.2022.102311. Epub 2022 Nov 12.
Crop yield and global food security are under constant threat from plant pathogens with the potential to cause epidemics. Traditional breeding for disease resistance can be too slow to counteract these emerging threats, resulting in the need to retool the plant immune system using bioengineered made-to-order immune receptors. Efforts to engineer immune receptors have focused primarily on nucleotide-binding domain and leucine-rich repeat (NLR) immune receptors and proof-of-principles studies. Based upon a near-exhaustive literature search of previously engineered plant immune systems we distil five emerging principles in the design of bioengineered made-to-order plant NLRs and describe approaches based on other components. These emerging principles are anticipated to assist the functional understanding of plant immune receptors, as well as bioengineering novel disease resistance specificities.
作物产量和全球粮食安全一直受到具有流行潜力的植物病原体的威胁。传统的抗病性育种可能太慢，无法应对这些新出现的威胁，因此需要使用生物工程定制的免疫受体重新构建植物免疫系统。工程免疫受体的努力主要集中在核苷酸结合域和富含亮氨酸重复（NLR）免疫受体和原理验证研究上。基于对以前工程化植物免疫系统的近乎详尽的文献检索，我们总结了生物工程定制植物 NLR 设计中的五个新出现的原则，并描述了基于其他成分的方法。预计这些新出现的原则将有助于对植物免疫受体的功能理解，以及生物工程新的抗病特异性。
