AGC protein kinases play important roles in regulating plant growth, immunity, and cell death. However, the function of AGC in has not yet been clarified. In this study, 62 genes were identified, and these genes were distributed on 10 chromosomes and divided into six subfamilies. Analysis of gene structure and conserved motifs showed that the activation segment of genes was highly conserved, and genes of the same subfamily showed higher sequence and structural similarity. Collinearity analysis revealed that were more closely related to than to . Expression profile analysis revealed that were preferentially expressed in flowers and , , and were preferentially expressed in the stigma; the expression of these genes was significantly upregulated after self-incompatibility pollination, and the expression of and was significantly upregulated after cross-pollination. In addition, several typical -elements involved in the stress response were identified in promoters. The expression levels of and significantly varied under different types of abiotic stress. Collectively, we identified that , and have the greatest potential in regulating pollen-pistil interaction and abiotic stress tolerance, respectively. Our findings will aid future functional investigations of in .