Rombach Helen, Alon Haguy, Shapiro Orr H, Elad Yigal, Kleiman Maya
Department of Agriculture and Horticulture, Humboldt University Zu Berlin, Berlin, Germany.
Institute of Plant Sciences, Department of Vegetables and Field Crops, Agricultural Research Organization (Volcani Center), Rishon Lezion, Israel.
Front Plant Sci. 2022 Oct 27;13:1023502. doi: 10.3389/fpls.2022.1023502. eCollection 2022.
For some pathogenic fungi, sensing surface topography is part of their infection strategy. Their directional growth and transformation to a new developmental stage is influenced by contact with topographic features, which is referred to as thigmo-response, the exact functionality of which is not fully understood. Research on thigmo-responses is often performed on biomimetically patterned surfaces (BPS). Polydimethylsiloxane (PDMS) is especially suitable for fabrication of BPS. Here, we used synthetic BPS surfaces, mimicking tomato leaf surface, made from PDMS with the pathogenic fungus to study the influence of structural features of the leaf surface on the fungus behavior. As a control, a PDMS surface without microstructure was fabricated to maintain the same chemical properties. Pre-penetration processes of , including the distribution of conidia on the surface, germination, and germ tube growth were observed on both leaf-patterned and flat PDMS. Microstructure affected the location of immediate attachment of conidia. Additionally, the microstructure of the plant host stimulated the development of germ tube in , at a higher rate than that observed on flat surface, suggesting that microstructure plays a role in fungus attachment and development.
对于一些致病真菌而言，感知表面形貌是其感染策略的一部分。它们的定向生长以及向新发育阶段的转变会受到与形貌特征接触的影响，这被称为触壁反应，其确切功能尚未完全明晰。关于触壁反应的研究通常在仿生图案化表面（BPS）上进行。聚二甲基硅氧烷（PDMS）特别适合用于制造BPS。在此，我们使用由PDMS制成的模仿番茄叶表面的合成BPS表面，结合致病真菌来研究叶表面结构特征对真菌行为的影响。作为对照，制作了一个没有微观结构的PDMS表面以保持相同的化学性质。在有叶图案的和扁平的PDMS上都观察到了该真菌的穿透前过程，包括分生孢子在表面的分布、萌发以及芽管生长。微观结构影响了分生孢子直接附着的位置。此外，植物宿主的微观结构以高于在平坦表面观察到的速率刺激了该真菌芽管的发育，这表明微观结构在真菌附着和发育中发挥作用。
