Jouriani Fatemeh Haririzadeh, Torkamaneh Mahdi, Torfeh Mahnaz, Ashrafian Fatemeh, Aghamohammad Shadi, Rohani Mahdi
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Clinical Research Department, Pasteur Institute of Iran, Tehran, Iran.
Sci Rep. 2025 Jul 11;15(1):25006. doi: 10.1038/s41598-025-09596-6.
Probiotics are beneficial microorganisms that modulate various signaling pathways to improve human health status. In this study we aimed to evaluate the precise molecular effects of Lactobacillus spp., Bifidobacterium spp., and a mixture of our native potential probiotics on the autophagy signaling pathway during the presence of inflammation. The evaluation of autophagy gene expression was performed after exposing the HT -29 cell line with the sonicated pathogens and probiotics, before and simultaneously with inflammation induction by quantitative real-time polymerase chain reaction (qPCR) and cytokine assays. The results of the current study showed that our native potential probiotic cocktails could upregulate the expression level of the autophagy genes including pik3c3, atg14, beclin, pik3r4, atg5, atg16, atg7, and atg3 compared with sonicated pathogen treatments, and also these native potential probiotic strains could exert anti-inflammatory effects, especially before inflammation induction. In conclusion, our native potential probiotic cocktail indicated the preventive and therapeutic effect on inflammation, but our selected probiotics could affect autophagy genes stronger before inflammation compared to expose simultaneously with inflammation. Therefore, the administration of probiotics as a prophylactic agent with the least side effects could be considered a suitable treatment for patients with inflammatory-related disease, even before or at the beginning of inflammation.
益生菌是有益微生物，可调节各种信号通路以改善人类健康状况。在本研究中，我们旨在评估乳酸杆菌属、双歧杆菌属以及我们本地潜在益生菌混合物在炎症存在期间对自噬信号通路的精确分子作用。在用超声处理过的病原体和益生菌处理HT -29细胞系后，在通过定量实时聚合酶链反应（qPCR）和细胞因子检测诱导炎症之前及同时，对自噬基因表达进行评估。当前研究结果表明，与超声处理过的病原体处理相比，我们本地潜在的益生菌混合物可上调自噬基因包括pik3c3、atg14、beclin、pik3r4、atg5、atg16、atg7和atg3的表达水平，并且这些本地潜在的益生菌菌株还可发挥抗炎作用，尤其是在炎症诱导之前。总之，我们本地潜在的益生菌混合物对炎症具有预防和治疗作用，但与在炎症同时暴露相比，我们所选的益生菌在炎症之前对自噬基因的影响更强。因此，即使在炎症之前或开始时，将益生菌作为副作用最小的预防剂给药也可被认为是炎症相关疾病患者的合适治疗方法。
