Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
Gastrointestinal and Liver Disease Research Center, Firoozgar Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Iran Biomed J. 2022 Sep 1;26(5):350-6. doi: 10.52547/ibj.3772.
Inflammatory bowel disease is a chronic inflammatory disease of the gastrointestinal tract. The gut microbiota is an important factor in the pathogenesis of inflammatory bowel disease (IBD). Due to a link between the gut microbiota and IBD, studying microbiota changes using an accurate, sensitive and rapid method for detection of the disease seems necessary. This study aimed to compare the composition of gut microbiota in three groups of people, including IBD patients, cured Inflammatory bowel disease (CIBD), and healthy groups.
For this study, 45 stool samples (15 from each group) were collected. Using real-time PCR, the abundance of 11 bacterial 16S rRNA gene sequences was examined.
In the IBD group, the number of three bacterial phyla, including Firmicutes, Actinobacteria, and Bacteroidetes, decreased (p < 0.01, p < 0.01, and p < 0.001, respectively), while the population of γ-Proteobacteria increased significantly (p < 0.0001). In the CIBD group, the number of Actinobacteria enhanced (p < 0.01), but that of Bacteroidetes and Firmicutes decreased (p < 0.01, and p < 0.05, respectively).
Findings of this study indicate that decrease in Firmicutes and increase in γ-Proteobacteria could be used as an indicator of IBD instead of employing invasive and costly detection methods such as colonoscopy and other tests.
炎症性肠病是一种胃肠道的慢性炎症性疾病。肠道微生物群是炎症性肠病（IBD）发病机制的重要因素。由于肠道微生物群与 IBD 之间存在联系，因此使用准确、敏感和快速的疾病检测方法来研究微生物群的变化似乎是必要的。本研究旨在比较三组人群（包括 IBD 患者、治愈的炎症性肠病（CIBD）患者和健康人群）的肠道微生物群组成。
本研究共采集了 45 份粪便样本（每组 15 份）。使用实时 PCR 检测了 11 种细菌 16S rRNA 基因序列的丰度。
在 IBD 组中，包括厚壁菌门、放线菌门和拟杆菌门在内的三种细菌门的数量减少（p < 0.01、p < 0.01 和 p < 0.001），而γ-变形菌门的数量显著增加（p < 0.0001）。在 CIBD 组中，放线菌门的数量增加（p < 0.01），但拟杆菌门和厚壁菌门的数量减少（p < 0.01 和 p < 0.05）。
本研究结果表明，厚壁菌门减少和γ-变形菌门增加可作为 IBD 的指标，而无需采用侵入性和昂贵的检测方法，如结肠镜检查和其他检测。
