Serov Dmitriy A, Baimler Ilya V, Burmistrov Dmitriy E, Baryshev Alexey S, Yanykin Denis V, Astashev Maxim E, Simakin Alexander V, Gudkov Sergey V
Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilova St., 119991 Moscow, Russia.
Polymers (Basel). 2022 Nov 12;14(22):4880. doi: 10.3390/polym14224880.
The bacterial contamination of cutting boards and other equipment in the meat processing industry is one of the key reasons for reducing the shelf life and consumer properties of products. There are two ways to solve this problem. The first option is to create coatings with increased strength in order to prevent the formation of micro damages that are favorable for bacterial growth. The second possibility is to create materials with antimicrobial properties. The use of polytetrafluoroethylene (PTFE) coatings with the addition of metal oxide nanoparticles will allow to the achieving of both strength and bacteriostatic effects at the same time. In the present study, a new coating based on PTFE and FeO nanoparticles was developed. FeO nanoparticles were synthesized by laser ablation in water and transferred into acetone using the developed procedures. An acetone-based colloidal solution was mixed with a PTFE-based varnish. Composites with concentrations of FeO nanoparticles from 0.001-0.1% were synthesized. We studied the effect of the obtained material on the generation of ROS (hydrogen peroxide and hydroxyl radicals), 8-oxoguanine, and long-lived active forms of proteins. It was found that PTFE did not affect the generation of all the studied compounds, and the addition of FeO nanoparticles increased the generation of HO and hydroxyl radicals by up to 6 and 7 times, respectively. The generation of 8-oxoguanine and long-lived reactive protein species in the presence of PTFE/FeO NPs at 0.1% increased by 2 and 3 times, respectively. The bacteriostatic and cytotoxic effects of the developed material were studied. PTFE with the addition of FeO nanoparticles, at a concentration of 0.001% or more, inhibited the growth of by 2-5 times compared to the control or PTFE without NPs. At the same time, PTFE, even with the addition of 0.1% FeO nanoparticles, did not significantly impact the survival of eukaryotic cells. It was assumed that the resulting composite material could be used to cover cutting boards and other polymeric surfaces in the meat processing industry.
肉类加工业中砧板及其他设备的细菌污染是缩短产品保质期和降低产品消费性能的关键原因之一。解决这个问题有两种方法。第一种选择是制造强度更高的涂层，以防止形成有利于细菌生长的微损伤。第二种可能性是制造具有抗菌性能的材料。添加金属氧化物纳米颗粒的聚四氟乙烯（PTFE）涂层的使用将能够同时实现强度和抑菌效果。在本研究中，开发了一种基于PTFE和FeO纳米颗粒的新型涂层。FeO纳米颗粒通过水中激光烧蚀合成，并使用所开发的程序转移到丙酮中。将丙酮基胶体溶液与PTFE基清漆混合。合成了FeO纳米颗粒浓度为0.001 - 0.1%的复合材料。我们研究了所得材料对活性氧（过氧化氢和羟基自由基）、8 - 氧代鸟嘌呤和蛋白质长寿命活性形式生成的影响。发现PTFE不影响所有研究化合物的生成，而添加FeO纳米颗粒分别使羟基和羟基自由基的生成增加了6倍和7倍。在0.1%的PTFE/FeO纳米颗粒存在下，8 - 氧代鸟嘌呤和长寿命反应性蛋白质物种的生成分别增加了2倍和3倍。研究了所开发材料的抑菌和细胞毒性作用。添加FeO纳米颗粒的PTFE，浓度为0.001%或更高时，与对照或不含纳米颗粒的PTFE相比，抑制生长2 - 5倍。同时，即使添加0.1% FeO纳米颗粒的PTFE也不会对真核细胞的存活产生显著影响。据推测，所得复合材料可用于覆盖肉类加工业中的砧板和其他聚合物表面。
