Weill Cornell Medicine, New York, NY, USA.
Institute for Computational Biomedicine, New York, NY, USA.
Cell Rep Methods. 2022 Oct 31;2(11):100325. doi: 10.1016/j.crmeth.2022.100325. eCollection 2022 Nov 21.
Single-cell RNA sequencing (scRNA-seq) and spatially resolved transcriptomics (SRT) have experienced rapid development in recent years. The findings of spaceflight-based scRNA-seq and SRT investigations are likely to improve our understanding of life in space and our comprehension of gene expression in various cell systems and tissue dynamics. However, compared to their Earth-based counterparts, gene expression experiments conducted in spaceflight have not experienced the same pace of development. Out of the hundreds of spaceflight gene expression datasets available, only a few used scRNA-seq and SRT. In this perspective piece, we explore the growing importance of scRNA-seq and SRT in space biology and discuss the challenges and considerations relevant to robust experimental design to enable growth of these methods in the field.
单细胞 RNA 测序 (scRNA-seq) 和空间分辨转录组学 (SRT) 近年来发展迅速。基于太空的 scRNA-seq 和 SRT 研究的发现可能会增进我们对太空生活的理解，以及对各种细胞系统和组织动态中基因表达的认识。然而，与基于地球的研究相比，太空飞行中的基因表达实验并没有经历同样的发展速度。在数百个可用的太空飞行基因表达数据集中，只有少数使用了 scRNA-seq 和 SRT。在这篇观点文章中，我们探讨了 scRNA-seq 和 SRT 在太空生物学中的重要性，并讨论了与稳健的实验设计相关的挑战和考虑因素，以促进这些方法在该领域的发展。
