Curova Katarina, Novotny Martin, Ambro Lubos, Kamlarova Anna, Lovayova Viera, Hrabovsky Vladimir, Siegfried Leonard, Jarcuska Pavol, Jarcuska Peter, Toporova Annamaria
Department of Medical and Clinical Microbiology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Kosice, Slovakia.
Department of Infectology and Travel Medicine, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Rastislavova 43, 04190 Kosice, Slovakia.
Pathogens. 2023 Mar 8;12(3):430. doi: 10.3390/pathogens12030430.
Dysbiosis of the gut microbiota, caused by antibiotics, plays a key role in the establishment of CD). Toxin-producing strains are involved in the pathogenesis of infection (CDI), one of the most common hospital-acquired infections. We cultured a total of 84 isolates from stool samples of patients hospitalized at Louis Pasteur University Hospital in Kosice, Slovakia, that were suspected of CDI and further characterized by molecular methods. The presence of genes encoding toxin A, toxin B, and binary toxin was assessed by toxin-specific PCR. CD ribotypes were detected using capillary-based electrophoresis ribotyping. A total of 96.4% of CD isolates carried genes encoding toxins A and B, and 54.8% of them were positive for the binary toxin. PCR ribotyping showed the presence of three major ribotypes: RT 176 (n = 40, 47.6%); RT 001 (n = 23, 27.4%); and RT 014 (n = 7, 8.3%). Ribotype 176 predominated among clinical CD isolates in our hospital. The proportion of RT 176 and RT 001 in four hospital departments with the highest incidence of CDI cases was very specific, pointing to local CDI outbreaks. Based on our data, previous use of antibiotics represents a significant risk factor for the development of CDI in patients over 65 years of age.
由抗生素引起的肠道微生物群失调在艰难梭菌感染（CDI）的发生中起关键作用。产毒素菌株参与了CDI（最常见的医院获得性感染之一）的发病机制。我们从斯洛伐克科希策的路易·巴斯德大学医院住院患者的粪便样本中总共培养了84株疑似CDI的分离株，并通过分子方法进行了进一步鉴定。通过毒素特异性PCR评估编码毒素A、毒素B和二元毒素的基因的存在情况。使用基于毛细管的电泳核糖分型检测CD核糖型。总共96.4%的CD分离株携带编码毒素A和B的基因，其中54.8%的分离株二元毒素呈阳性。PCR核糖分型显示存在三种主要核糖型：RT 176（n = 40，47.6%）；RT 001（n = 23，27.4%）；和RT 014（n = 7，8.3%）。核糖型176在我院临床CD分离株中占主导地位。在CDI病例发病率最高的四个医院科室中，RT 176和RT 001的比例非常特殊，表明存在局部CDI暴发。根据我们的数据，既往使用抗生素是65岁以上患者发生CDI的一个重要危险因素。
