Idris Sanaa M, Ali Eva A, Elmagzoub Wisal A, Okuni Julius B, Mukhtar Mohamed E, Ojok Lonzy, Eltayeb ElSagad, Abd El Wahed Ahmed, Eltom Kamal H, Gameel Ahmed A
Department of Animal Health and Safety of Animal Products, Institute for Studies and Promotion of Animal Exports, University of Khartoum, Shambat 13314, Khartoum North, Sudan.
Department of Pathology, Faculty of Veterinary Medicine, University of Khartoum, Shambat 13314, Khartoum North, Sudan.
Animals (Basel). 2022 Nov 27;12(23):3312. doi: 10.3390/ani12233312.
Paratuberculosis (PTB) has been reported in the Sudan in cattle and goats for more than 50 years but has never been reported in sheep. However, suspicion of the disease in a breeding flock of sheep in Khartoum North locality was made due to a history of unknown cause of loss of weight. Blood and faecal samples were collected from all animals (N = 59): harvested sera were tested for anti- subsp. (MAP) antibodies by Enzyme Linked Immunosorbent Assay (ELISA); faeces were screened for acid-fast bacilli by Ziehl-Neelsen staining, tested for MAP DNA by recombinase polymerase amplification (RPA) and some faecal samples were cultured for MAP isolation. Typical MAP acid-fast bacilli were seen in 10.2% (6/59) of the faecal smears, 37.5% of the tested faecal samples (12/32) were positive for MAP DNA and only 3 (5.1%) animals were seropositive for MAP. MAP positive cultures were obtained from 2 out the 6 samples showing typical MAP acid-fast bacilli; the isolates were confirmed by real-time PCR and sequencing. As sheep are animals of utmost economic importance as the main export animals for the country, this first report of ovine PTB warrants special considerations and more investigations for planning control programmes of the disease.
在苏丹，牛和山羊的副结核病（PTB）已有50多年的报道，但绵羊中从未有过相关报道。然而，由于存在不明原因体重减轻的病史，北喀土穆地区一群繁殖绵羊被怀疑感染了这种疾病。从所有动物（N = 59）采集了血液和粪便样本：采集的血清通过酶联免疫吸附测定（ELISA）检测抗副结核分枝杆菌亚种（MAP）抗体；粪便通过萋-尼氏染色筛选抗酸杆菌，通过重组酶聚合酶扩增（RPA）检测MAP DNA，部分粪便样本进行MAP分离培养。在10.2%（6/59）的粪便涂片中可见典型的MAP抗酸杆菌，37.5%的检测粪便样本（12/32）MAP DNA呈阳性，仅3只动物（5.1%）MAP血清学检测呈阳性。从6份显示典型MAP抗酸杆菌的样本中的2份获得了MAP阳性培养物；分离株通过实时PCR和测序得到确认。由于绵羊作为该国主要出口动物具有极其重要的经济意义，绵羊PTB的这一首次报道值得特别关注，并需要开展更多调查以制定该疾病的防控计划。
