Aukrust L, Almeland T L, Aas K
Scand J Immunol. 1978;8(5):421-7. doi: 10.1111/j.1365-3083.1978.tb00537.x.
The major allergen from codfish (DS 22) was used to study specific and non-specific radiostaining in crossed radioimmunoelectrophoresis (CRIE). Various modifications of CRIE experiments were performed including experiments where immunoprecipitates in agarose were transferred to test tubes and the radioactivity was measured in a gammacounter. The degree of non-specific binding of IgE and 125 I-anti-IgE to the percipitates was low. A linear relation was found between the amount of human serum IgE antibody used in CRIE and the degree of radiostaining obtained. Increasing the amount of DS 22 in the precipitates from 0.31 to 2.5 microgram resulted in a moderate increase in the uptake of 125 I-anti-IgE, whereas further increase of the amount of DS 22 (5--10 microgram) resulted in reduced uptake of isotope. Serum from a patient containing IgE antibodies to rabbit serum gave rise to radiostaining over the entire antibody-containing gel area, and it is suggested that such human sera be avoided when using CRIE.
鳕鱼主要过敏原（DS 22）被用于研究交叉放射免疫电泳（CRIE）中的特异性和非特异性放射染色。进行了CRIE实验的各种改进，包括将琼脂糖中的免疫沉淀物转移到试管中并在γ计数器中测量放射性的实验。IgE和125I-抗IgE与沉淀物的非特异性结合程度较低。在CRIE中使用的人血清IgE抗体量与获得的放射染色程度之间发现了线性关系。将沉淀物中DS 22的量从0.31微克增加到2.5微克导致125I-抗IgE摄取量适度增加，而DS 22量的进一步增加（5 - 10微克）导致同位素摄取量减少。含有针对兔血清的IgE抗体的患者血清在整个含抗体凝胶区域产生放射染色，建议在使用CRIE时避免使用此类人血清。
