He Wenxiu, Xing Yixiao, Li Chunlei, Zhou Peiru, Hu Xiaosheng, Hua Hong, Wei Pan
Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, Beijing 100081, China.
Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Diagnostics (Basel). 2022 Dec 6;12(12):3058. doi: 10.3390/diagnostics12123058.
Pemphigus vulgaris (PV) is a potentially fatal autoimmune bullous disease. The role of microRNA (miRNA, miR) in the diagnosis and pathogenesis of PV remains unknown. This study aims to provide potential miRNA biomarkers for PV diagnosis and therapy options.
Serum samples were obtained from 22 PV patients, 15 mucous membrane pemphigoid (MMP) patients, and 10 normal controls (NC). Total RNA was extracted from the serum samples, and 12 selected miRNAs were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Bioinformatic analyses including target gene prediction and enrichment analysis were performed.
Twelve miRNAs were increased in the serum of the PV group compared with the NC group, in which six miRNAs had good efficacy to diagnose PV from MMP with the area under the receiver operator characteristic curves of 0.970 to 0.988. A series test for the combination of miR-584-5p and miR-155-5p reached the sensitivity and specificity of 95.5% and 100%. Bioinformatic analysis revealed target gene enrichment in the cell adhesion pathways, immune-relating pathways, and P38 mitogen-activated protein kinases signaling pathway.
The study provides new insights and targets of miRNAs for the precise diagnosis and the exploration of pathogenesis for PV, which may serve as a reference for further research into autoimmune bullous diseases.
寻常型天疱疮（PV）是一种具有潜在致命性的自身免疫性大疱性疾病。微小RNA（miRNA，miR）在PV诊断和发病机制中的作用尚不清楚。本研究旨在为PV的诊断和治疗方案提供潜在的miRNA生物标志物。
采集22例PV患者、15例黏膜类天疱疮（MMP）患者和10例正常对照（NC）的血清样本。从血清样本中提取总RNA，通过定量实时聚合酶链反应（qRT-PCR）检测12种选定的miRNA。进行了包括靶基因预测和富集分析在内的生物信息学分析。
与NC组相比，PV组血清中12种miRNA升高，其中6种miRNA对PV与MMP的诊断效能良好，受试者操作特征曲线下面积为0.970至0.988。miR-584-5p和miR-155-5p联合检测的敏感度和特异度分别达到95.5%和100%。生物信息学分析显示靶基因在细胞黏附途径、免疫相关途径和P38丝裂原活化蛋白激酶信号通路中富集。
本研究为PV的精准诊断和发病机制探索提供了新的miRNA见解和靶点，可为自身免疫性大疱性疾病的进一步研究提供参考。
