Horticulture Department, Faculty of Horticulture and Plant Protection Science, Inner Mongolia Agricultural University, Hohhot 010019, China.
Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China.
Genes (Basel). 2022 Dec 6;13(12):2296. doi: 10.3390/genes13122296.
β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 members were identified in yam ( Thunb.). A novel β-amylase gene , (named ), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of were characterized and found to all be higher than in Bikeqi. overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and β-amylase. Under cold treatment, we observed the induced upregulation of and lower starch content and malondialdehyde (MDA) accumulation than in WT plants. In conclusion, these results demonstrate that overexpression plays an advanced role in cold tolerance, at least in part by raising the levels of soluble sugars that are capable of acting as osmolytes or antioxidants.
β-淀粉酶（BAM）在植物发育和应对非生物胁迫中起着重要作用。本研究在山药中鉴定了 5 个成员。从山药品种 Bikeqi 和 Dahechangyu 中分离出一种新型的β-淀粉酶基因，（命名为）。的开放阅读框（ORF）长 2806bp，编码 543 个氨基酸。亚细胞定位分析表明，DoBAM1 定位于细胞膜和细胞质。在山药品种 Dahechangyu 中，对淀粉含量、β-淀粉酶活性和表达进行了表征，发现均高于 Bikeqi。在烟草中过表达显示可促进可溶性糖和叶绿素含量的积累，并提高过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和β-淀粉酶的活性。在冷处理下，观察到诱导上调，淀粉含量和丙二醛（MDA）积累低于 WT 植物。总之，这些结果表明，过表达在耐寒性中起重要作用，至少部分是通过提高能够作为渗透调节剂或抗氧化剂的可溶性糖水平。
