益生菌菌株EPS DA-LAIM产生的胞外多糖的结构分析及益生元活性
Structural analysis and prebiotic activity of exopolysaccharide produced by probiotic strain EPS DA-LAIM.
作者信息
Kang Min Joo, Jeong Huijin, Kim Suin, Shin Jaein, Song Youngbo, Lee Byung-Hoo, Park Hyoung-Geun, Lee Tae-Ho, Jiang Hai-Hua, Han Young-Sun, Lee Bong-Gyeong, Lee Ho-Jin, Park Min-Ju, Park Young-Seo
机构信息
Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.
Research Laboratory, Dong-A Pharmaceutical Co., Ltd., Yongin, 17073 Republic of Korea.
出版信息
Food Sci Biotechnol. 2022 Dec 1;32(4):517-529. doi: 10.1007/s10068-022-01213-w. eCollection 2023 Mar.
UNLABELLED
Exopolysaccharide (EPS)-producing EPS DA-LAIM was isolated from healthy human feces, the structure of purified EPS from the strain was analyzed, and its prebiotic activity was evaluated. The EPS from EPS DA-LAIM is a glucomannan-type heteropolysaccharide with a molecular weight of 407-1007 kDa, and its structure comprises 2-mannosyl, 6-mannosyl, and 2,6-mannosyl residues. The purified EPS promoted the growth of representative lactic acid bacteria and bifidobacterial strains. EPS DA-LAIM increased nitric oxide production in RAW 264.7 macrophage cells, indicating its immunostimulatory activity. EPS DA-LAIM also exhibited high gastrointestinal tract tolerance, gut adhesion ability, and antioxidant activity. These results suggest that EPS from EPS DA-LAIM is a potentially useful prebiotic material, and EPS DA-LAIM could be applied as a probiotic candidate.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01213-w.
未标记
从健康人粪便中分离出产生胞外多糖（EPS）的EPS DA-LAIM，分析了该菌株纯化EPS的结构，并评估了其益生元活性。来自EPS DA-LAIM的EPS是一种分子量为407-1007 kDa的葡甘露聚糖型杂多糖，其结构包含2-甘露糖基、6-甘露糖基和2,6-甘露糖基残基。纯化的EPS促进了代表性乳酸菌和双歧杆菌菌株的生长。EPS DA-LAIM增加了RAW 264.7巨噬细胞中一氧化氮的产生，表明其具有免疫刺激活性。EPS DA-LAIM还表现出高胃肠道耐受性、肠道黏附能力和抗氧化活性。这些结果表明，来自EPS DA-LAIM的EPS是一种潜在有用的益生元材料，并且EPS DA-LAIM可作为益生菌候选物应用。
补充信息
在线版本包含可在10.1007/s10068-022-01213-w获取的补充材料。
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