Naderi Elham, Khajavi Rad Abolfazl, Nazari Somayeh, Khazaei Majid, Shahraki Samira, Hosseinian Sara
Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2022 Nov-Dec;12(6):638-648. doi: 10.22038/AJP.2022.20921.
Rhabdomyolysis is a life-threatening disease caused by releasing myoglobin from injured myocytes, which results in acute kidney injury. In this study, the effect of aqueous-alcoholic extract of (NS) and thymoquinone (TQ) on rhabdomyolysis-induced kidney damage in rats was investigated.
There were five groups of rats (n=8): Control, rhabdomyolysis, rhabdomyolysis treated with NS aqueous-alcoholic extract (200 and 400 mg/kg) and TQ (15 mg/kg). Treatments were given for 7 days (two days before and four days after glycerol injection). Glycerol was injected intramuscularly on the third day of the experiment for induction of rhabdomyolysis. Renal function parameters on the first, fourth, and seventh days of the experiment and renal oxidative stress and histological changes at the end of this study were assessed.
Glycerol injection caused a significant increase in serum level of urea, creatinine, creatine phosphokinase, urine output and tissue MDA compared to the control animals (p<0.05-0.001). Administration of NS extract and TQ significantly decreased serum urea and creatinine on days 4 and 7, creatine phosphokinase on day 4, and urine output on day 7 compared to the rhabdomyolysis group (p<0.05-0.001). Compared to the rhabdomyolysis group, treatment with NS extract and TQ improved kidney histological abnormalities (p<0.01-0.001). The catalase enzyme activity in the group treated with NS 400 mg/kg and thiol content in the NS 400 mg/kg and TQ groups were significantly higher than those of the rhabdomyolysis group (p<0.05-0.01).
extract and to some extent TQ protect the kidney from rhabdomyolysis-induced injury.
横纹肌溶解症是一种由受损肌细胞释放肌红蛋白引起的危及生命的疾病，可导致急性肾损伤。本研究探讨了黑种草子水醇提取物（NS）和百里醌（TQ）对大鼠横纹肌溶解症所致肾损伤的影响。
将大鼠分为五组（n = 8）：对照组、横纹肌溶解症组、NS水醇提取物（200和400 mg/kg）治疗的横纹肌溶解症组以及TQ（15 mg/kg）治疗的横纹肌溶解症组。给药7天（甘油注射前两天和后四天）。在实验第三天肌肉注射甘油以诱导横纹肌溶解症。评估实验第1、4和7天的肾功能参数以及本研究结束时的肾脏氧化应激和组织学变化。
与对照动物相比，注射甘油导致血清尿素、肌酐、肌酸磷酸激酶水平、尿量和组织丙二醛显著升高（p<0.05 - 0.001）。与横纹肌溶解症组相比，给予NS提取物和TQ可使第4天和第7天的血清尿素和肌酐、第4天的肌酸磷酸激酶以及第7天的尿量显著降低（p<0.05 - 0.001）。与横纹肌溶解症组相比，NS提取物和TQ治疗改善了肾脏组织学异常（p<0.01 - 0.001）。NS 400 mg/kg组的过氧化氢酶活性以及NS 400 mg/kg组和TQ组的巯基含量显著高于横纹肌溶解症组（p<0.05 - 0.01）。
NS提取物以及在一定程度上TQ可保护肾脏免受横纹肌溶解症所致损伤。
