ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38043 Grenoble, France.
EMBL Grenoble, 71 Avenue des Martyrs, 38042 Grenoble, France.
J Synchrotron Radiat. 2023 Jan 1;30(Pt 1):258-266. doi: 10.1107/S1600577522011286.
As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF's BM29 BioSAXS beamline was subject to a significant upgrade and refurbishment. In addition to the replacement of the beamline's original bending magnet source by a two-pole wiggler, leading to an increase in brilliance by a factor of 60, the sample environment of the beamline was almost completely refurbished: a vacuum-compatible Pilatus3 X 2M with a sensitive area of 253.7 mm × 288 mm and frame rates up to 250 Hz was installed, increasing the active area available and thus the q-scaling of scattering images taken; the sample changer was replaced with an upgraded version, allowing more space for customizable sample environments and the installation of two new sample exposure units; the software associated with the beamline was also renewed. In addition, the layout and functionality of the BSXCuBE3 (BioSAXS Customized Beamline Environment) data acquisition software was redesigned, providing an intuitive `user first' approach for inexperienced users, while at the same time maintaining more powerful options for experienced users and beamline staff. Additional features of BSXCuBE3 are queuing of samples; either consecutive sample changer and/or SEC-SAXS (size-exclusion chromatography small-angle X-ray scattering) experiments, including column equilibration were also implemented. Automatic data processing and analysis are now managed via Dahu, an online server with upstream data reduction, data scaling and azimuthal integration built around PyFAI (Python Fast Azimuthal Integration), and data analysis performed using the open source FreeSAS. The results of this automated data analysis pipeline are displayed in ISPyB/ExiSAXS. The upgraded BM29 has been in operation since the post-EBS restart in September 2020, and here a full description of its new hardware and software characteristics together with examples of data obtained are provided.
作为其极端出色源（EBS）升级项目的一部分，ESRF 的 BM29 BioSAXS 光束线经历了重大升级和翻新。除了用两极摆动器替换光束线原始的弯曲磁铁源，从而使亮度提高了 60 倍之外，光束线的样品环境几乎完全翻新：安装了一个兼容真空的 Pilatus3 X 2M，其敏感面积为 253.7 mm×288 mm，帧率高达 250 Hz，增加了可用的活动面积，从而提高了散射图像的 q 比例；更换了升级后的样品更换器，为可定制的样品环境留出更多空间，并安装了两个新的样品曝光单元；还更新了与光束线相关的软件。此外，BSXCuBE3（BioSAXS 定制光束线环境）数据采集软件的布局和功能进行了重新设计，为没有经验的用户提供了直观的“以用户为中心”的方法，同时为有经验的用户和光束线工作人员保留了更强大的选项。BSXCuBE3 的其他功能包括样品排队；连续的样品更换器和/或 SEC-SAXS（尺寸排阻色谱小角 X 射线散射）实验，包括柱平衡也已实现。自动数据处理和分析现在通过 Dahu 进行管理，Dahu 是一个在线服务器，具有上游数据减少、数据缩放和围绕 PyFAI（Python 快速方位积分）的方位积分以及使用开源 FreeSAS 进行数据分析的功能。此自动化数据分析管道的结果显示在 ISPyB/ExiSAXS 中。升级后的 BM29 自 2020 年 9 月 EBS 重启以来一直在运行，本文提供了其新硬件和软件特性的完整描述，并提供了获得的数据示例。
