Center for Life Sciences, School of Life Sciences, State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan, Yunnan University, Kunming 650500, China.
STAR Protoc. 2023 Mar 17;4(1):101990. doi: 10.1016/j.xpro.2022.101990. Epub 2023 Jan 4.
The digestive system works by moving food through the gastrointestinal tract, which processes food into molecules that can be absorbed and utilized by the cells of the body. However, little is known about the signaling pathways that regulate food digestion. Here, by using the inedible bacteria (Staphylococcus saprophyticus)-worm culture system, we established a simple food digestion methodology, which is of low cost and high efficiency, to explore molecular mechanisms underlying food digestion in animals. For complete details on the use and execution of this protocol, please refer to Geng et al. (2022)..
消化系统通过将食物在胃肠道中移动来工作,胃肠道将食物分解成可以被身体细胞吸收和利用的分子。然而,人们对调节食物消化的信号通路知之甚少。在这里,我们使用不可食用的细菌(腐生葡萄球菌)-蠕虫培养系统,建立了一种简单、低成本、高效率的食物消化方法,以探索动物食物消化的分子机制。有关该方案使用和执行的完整详细信息,请参见 Geng 等人。(2022 年)。