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聚苯乙烯纳米颗粒在人肺细胞中的内吞作用、分布及胞吐作用

Endocytosis, Distribution, and Exocytosis of Polystyrene Nanoparticles in Human Lung Cells.

作者信息

Liu Yuan-Yuan, Liu Jie, Wu Hao, Zhang Qiangqiang, Tang Xue-Rui, Li Dan, Li Chen-Si, Liu Yuanfang, Cao Aoneng, Wang Haifang

机构信息

Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 200444, China.

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

出版信息

Nanomaterials (Basel). 2022 Dec 24;13(1):84. doi: 10.3390/nano13010084.

DOI:10.3390/nano13010084
PMID:36615994
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9824409/
Abstract

Nanoplastics, one component of plastic pollution, can enter human bodies via inhalation and thus threaten human health. However, the knowledge about the uptake and exocytosis of nanoplastics in cells of human lung organs is still very limited. Herein, we investigated the endocytosis, distribution, and exocytosis of polystyrene nanoparticles (PS NPs) of 50 nm (G50PS) and 100 nm (R100PS) in A549 cells and BEAS-2B cells. We found that both the cellular uptake of PS NPs increased positively with exposure time and dose, and A549 cells ingested more PS NPs than BEAS-2B cells did. In addition, the intracellular content of G50PS was higher than that of R100PS except at a higher dose and longer time. The ingested PS NPs were distributed mainly in lysosomes, while many G50PS appeared around the cell membrane, and R100PS also accumulated in mitochondria in BEAS-2B cells. As for the exocytosis, R100PS was more difficult to excrete than G50PS. Lysosomes in A549 cells and actin and microtubule in BEAS-2B cells were involved in the exocytosis of the PS NPs. These findings provide detailed information about the translocation of nanoplastics in lung cells, which is valuable for the safety assessment of nanoplastics in the environment.

摘要

纳米塑料作为塑料污染的一个组成部分，可通过吸入进入人体，从而威胁人类健康。然而，关于纳米塑料在人肺器官细胞中的摄取和胞吐作用的了解仍然非常有限。在此，我们研究了50纳米（G50PS）和100纳米（R100PS）的聚苯乙烯纳米颗粒在A549细胞和BEAS-2B细胞中的内吞作用、分布和胞吐作用。我们发现，PS NPs的细胞摄取量均随暴露时间和剂量呈正增加，且A549细胞摄取的PS NPs比BEAS-2B细胞更多。此外，除了在较高剂量和较长时间外，G50PS的细胞内含量高于R100PS。摄取的PS NPs主要分布在溶酶体中，而许多G50PS出现在细胞膜周围，R100PS在BEAS-2B细胞的线粒体中也有积累。至于胞吐作用，R100PS比G50PS更难排出。A549细胞中的溶酶体以及BEAS-2B细胞中的肌动蛋白和微管参与了PS NPs的胞吐作用。这些发现提供了关于纳米塑料在肺细胞中转运的详细信息，这对于环境中纳米塑料的安全性评估具有重要价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/3a7b1c64a74c/nanomaterials-13-00084-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/f42821597b73/nanomaterials-13-00084-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/98c535a473f4/nanomaterials-13-00084-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/a19ea919de4d/nanomaterials-13-00084-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/ba428d439f50/nanomaterials-13-00084-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/f1ec15f1b507/nanomaterials-13-00084-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/e7ecf2feb5a5/nanomaterials-13-00084-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/81595095dead/nanomaterials-13-00084-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/9376b2f63542/nanomaterials-13-00084-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/35ac77f8219a/nanomaterials-13-00084-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/3a7b1c64a74c/nanomaterials-13-00084-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/f42821597b73/nanomaterials-13-00084-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/98c535a473f4/nanomaterials-13-00084-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/a19ea919de4d/nanomaterials-13-00084-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/ba428d439f50/nanomaterials-13-00084-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/f1ec15f1b507/nanomaterials-13-00084-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/e7ecf2feb5a5/nanomaterials-13-00084-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/81595095dead/nanomaterials-13-00084-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/9376b2f63542/nanomaterials-13-00084-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/35ac77f8219a/nanomaterials-13-00084-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6070/9824409/3a7b1c64a74c/nanomaterials-13-00084-g010.jpg

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[6]
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本文引用的文献

[1]
Recognition and movement of polystyrene nanoplastics in fish cells.

Environ Pollut. 2023-1-1

[2]
Polystyrene Nanoplastics Induce Lung Injury via Activating Oxidative Stress: Molecular Insights from Bioinformatics Analysis.

Nanomaterials (Basel). 2022-10-7

[3]
Cytotoxicity and Genotoxicity of Polystyrene Micro- and Nanoplastics with Different Size and Surface Modification in A549 Cells.

Int J Nanomedicine. 2022

[4]
Amine-modified nanoplastics promote the procoagulant activation of isolated human red blood cells and thrombus formation in rats.

Part Fibre Toxicol. 2022-9-14

[5]
On the Cellular Uptake and Exocytosis of Carbon Dots─Significant Cell Type Dependence and Effects of Cell Division.

ACS Appl Bio Mater. 2022-8-31

[6]
Probing the Effect of Rigidity on the Cellular Uptake of Core-Shell Nanoparticles: Stiffness Effects are Size Dependent.

Small. 2022-9

[7]
Bioaccumulation of differently-sized polystyrene nanoplastics by human lung and intestine cells.

J Hazard Mater. 2022-10-5

[8]
Toxic effects of nanoplastics with different sizes and surface charges on epithelial-to-mesenchymal transition in A549 cells and the potential toxicological mechanism.

J Hazard Mater. 2022-5-15

[9]
The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes.

Nanomaterials (Basel). 2022-5-11

[10]
Consequences of nano and microplastic exposure in rodent models: the known and unknown.

Part Fibre Toxicol. 2022-4-21

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