Department of Physiology and Aging, College of Medicine, University of Florida, Gainesville, FL 32610, USA; Myology Institute, University of Florida, Gainesville, FL 32610, USA.
Department of Physiology and Aging, College of Medicine, University of Florida, Gainesville, FL 32610, USA; Myology Institute, University of Florida, Gainesville, FL 32610, USA; Claude D. Pepper Older Americans Independence Center, University of Florida, Gainesville, FL 32610, USA.
Cell Rep. 2023 Jan 31;42(1):111982. doi: 10.1016/j.celrep.2022.111982. Epub 2023 Jan 9.
Cellular circadian clocks direct a daily transcriptional program that supports homeostasis and resilience. Emerging evidence has demonstrated age-associated changes in circadian functions. To define age-dependent changes at the systems level, we profile the circadian transcriptome in the hypothalamus, lung, heart, kidney, skeletal muscle, and adrenal gland in three age groups. We find age-dependent and tissue-specific clock output changes. Aging reduces the number of rhythmically expressed genes (REGs), indicative of weakened circadian control. REGs are enriched for the hallmarks of aging, adding another dimension to our understanding of aging. Analyzing differential gene expression within a tissue at four different times of day identifies distinct clusters of differentially expressed genes (DEGs). Increased variability of gene expression across the day is a common feature of aged tissues. This analysis extends the landscape for understanding aging and highlights the impact of aging on circadian clock function and temporal changes in gene expression.
细胞生物钟指导着支持内稳态和弹性的每日转录程序。新出现的证据表明生物钟功能与年龄相关的变化。为了在系统水平上定义与年龄相关的变化，我们在三个年龄组中分析了下丘脑、肺、心脏、肾脏、骨骼肌和肾上腺的生物钟转录组。我们发现了与年龄相关的和组织特异性的生物钟输出变化。衰老减少了节律表达基因（REGs）的数量，表明生物钟控制减弱。REGs 富集了衰老的特征，为我们理解衰老增加了一个维度。在一天中的四个不同时间分析组织内的差异基因表达，确定了不同表达基因（DEGs）的不同聚类。全天基因表达的变异性增加是老年组织的一个共同特征。这种分析扩展了理解衰老的范围，并强调了衰老对生物钟功能和基因表达时间变化的影响。
