现场红细胞中乙酰胆碱酯酶的测定。
Measurement of acetylcholinesterase in erythrocytes in the field.
作者信息
Magnotti R A, Eberly J P, Quarm D E, McConnell R S
机构信息
Department of Internal Medicine, University of Cincinnati Medical Center, OH 45267-0585.
出版信息
Clin Chem. 1987 Oct;33(10):1731-5.
We describe here a field method we developed for colorimetry of erythrocytic acetylcholinesterase (EC 3.1.1.7) in capillary blood samples. Three stable, premixed assay reagents and de-ionized water (but no centrifuge or balance) are required. This method, adapted for a microplate format, is essentially that of Ellman et al. (Biochem Pharmacol 1961;7:88-95) as modified by George and Abernethy (Clin Chem 1983;29:365-8). Assays were quantified and corrected for hematocrit by using a battery-powered colorimeter with a silicon carbide (blue) light-emitting-diode source. Advantages over existing field methods include better portability, ruggedness, greater precision, and lower cost per sample.
我们在此描述一种我们开发的用于比色法测定毛细血管血样中红细胞乙酰胆碱酯酶（EC 3.1.1.7）的现场方法。该方法需要三种稳定的预混测定试剂和去离子水（但不需要离心机或天平）。这种适用于微孔板形式的方法本质上是由埃尔曼等人（《生物化学与药理学》，1961年；7：88 - 95）提出，并经乔治和阿伯内西修改（《临床化学》，1983年；29：365 - 368）的方法。使用带有碳化硅（蓝色）发光二极管光源的电池供电比色计对测定进行定量并针对血细胞比容进行校正。与现有现场方法相比，其优点包括更好的便携性、耐用性、更高的精度以及更低的每份样品成本。
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