Al-Qazzaz Haider H, Abdullah Bashar H, Museedi Omar S
Department of Pathology Al Yarmouk Teaching Hospital/Teaching Laboratories Baghdad Iraq.
Department of Oral Diagnosis, College of Dentistry University of Baghdad Baghdad Iraq.
Health Sci Rep. 2023 Jan 12;6(1):e1061. doi: 10.1002/hsr2.1061. eCollection 2023 Jan.
Odontogenic cysts and tumors often form hard and soft structures that resemble odontogenesis. It is well known that amyloid is produced in Pindborg tumors; however, it is still debatable whether it is also formed in other odontogenic tumors and cysts. This study aimed to detect the presence of amyloid in different odontogenic cysts and tumors in correlation to matrix proteins secreted during enamel formation; namely amelogenin and odontogenic ameloblast-associated protein.
This study included formalin fixed paraffin embedded tissue blocks of 106 different types of odontogenic cysts and tumors. Congo red and thioflavin T were performed to confirm the presence of amyloid; immunohistochemistry was used to detect amelogenin and odontogenic ameloblast-associated protein.
Amyloid was detected in pindborg tumors (conventional), adenomatoid odontogenic tumors, odontogenic fibroma (Amyloid variant), follicular solid and unicystic ameloblastomas, radicular cysts, dentigerous cysts, dentinogenic ghost cell odontogenic tumor, ameloblastic fibroma, calcifying odontogenic cyst, and primordial Odontogenic tumor. Amelogenin was detected in 95.3% of the cases, while odontogenic ameloblast-associated protein was detected in 93.4% of the cases. The association between odontogenic ameloblast-associated protein and amyloid was highly significant at < 0.01. However, there was no significant relationship between amelogenin and amyloid > 0.05.
Although pindborg tumor is the bonafide example of amyloid deposition in odontogenic tumors, this study concluded that amyloid may be deposited in traces to massive amounts in various odontogenic cysts and tumors, and it is significantly linked to odontogenic ameloblast-associated protein but not amelogenin matrix protein, since all amyloid cases were odontogenic ameloblast associated protein positive.
牙源性囊肿和肿瘤常形成类似牙胚发生的软硬结构。众所周知，在牙源性钙化上皮瘤中会产生淀粉样物质；然而，其在其他牙源性肿瘤和囊肿中是否也会形成仍存在争议。本研究旨在检测不同牙源性囊肿和肿瘤中淀粉样物质的存在情况，并与釉质形成过程中分泌的基质蛋白（即釉原蛋白和成釉细胞相关蛋白）进行关联分析。
本研究纳入了106例不同类型牙源性囊肿和肿瘤的福尔马林固定石蜡包埋组织块。采用刚果红和硫黄素T染色来确认淀粉样物质的存在；运用免疫组织化学方法检测釉原蛋白和成釉细胞相关蛋白。
在牙源性钙化上皮瘤（传统型）、腺样牙源性肿瘤、牙源性纤维瘤（淀粉样变亚型）、滤泡型实性及单囊性成釉细胞瘤、根端囊肿、含牙囊肿、牙本质生成性影细胞牙源性肿瘤、成釉细胞纤维瘤、钙化牙源性囊肿和原始牙源性肿瘤中检测到了淀粉样物质。95.3%的病例检测到了釉原蛋白，93.4%的病例检测到了成釉细胞相关蛋白。成釉细胞相关蛋白与淀粉样物质之间的关联在P<0.01时具有高度显著性。然而，釉原蛋白与淀粉样物质之间在P>0.05时无显著关系。
尽管牙源性钙化上皮瘤是牙源性肿瘤中淀粉样物质沉积的典型例子，但本研究得出结论，淀粉样物质可能在各种牙源性囊肿和肿瘤中微量至大量沉积，并且它与成釉细胞相关蛋白显著相关，而与釉原蛋白基质蛋白无关，因为所有淀粉样物质阳性的病例均为成釉细胞相关蛋白阳性。
