Wang Xiaoyu, Song Rijian, Johnson Melissa, A Sigen, He Zhonglei, Milne Cameron, Wang Xianqing, Lara-Sáez Irene, Xu Qian, Wang Wenxin
The Charles Institute of Dermatology, School of Medicine, University College Dublin, Dublin 4, Eircode D04 V1W8, Ireland.
Materials (Basel). 2021 Oct 11;14(20):5956. doi: 10.3390/ma14205956.
Due to their biodegradability and biocompatibility, chitosan-based hydrogels have great potential in regenerative medicine, with applications such as bacteriostasis, hemostasis, and wound healing. However, toxicity and high cost are problems that must be solved for chitosan-based hydrogel crosslinking agents such as formaldehyde, glutaraldehyde, and genipin. Therefore, we developed a biocompatible yet cost-effective chitosan-based hydrogel system as a candidate biomaterial to prevent infection during wound healing. The hydrogel was fabricated by crosslinking chitosan with dialdehyde chitosan (CTS-CHO) via dynamic Schiff-base reactions, resulting in a self-healable and injectable system. The rheological properties, degradation profile, and self-healable properties of the chitosan-based hydrogel were evaluated. The excellent antibacterial activity of the hydrogel was validated by a spread plate experiment. The use of Live/Dead assay on HEK 293 cells showed that the hydrogel exhibited excellent biocompatibility. The results demonstrate that the newly designed chitosan-based hydrogel is an excellent antibacterial wound dressing candidate with good biocompatibility.
由于其生物可降解性和生物相容性，基于壳聚糖的水凝胶在再生医学中具有巨大潜力，可用于抑菌、止血和伤口愈合等应用。然而，甲醛、戊二醛和京尼平之类基于壳聚糖的水凝胶交联剂存在毒性和成本高的问题。因此，我们开发了一种生物相容性好且成本效益高的基于壳聚糖的水凝胶系统，作为伤口愈合期间预防感染的候选生物材料。该水凝胶通过壳聚糖与二醛壳聚糖（CTS-CHO）经由动态席夫碱反应交联制备而成，形成了一种可自愈且可注射的系统。对基于壳聚糖的水凝胶的流变学性质、降解曲线和自愈性能进行了评估。通过平板涂布实验验证了水凝胶出色的抗菌活性。对HEK 293细胞进行的活/死检测表明，该水凝胶表现出出色的生物相容性。结果表明，新设计的基于壳聚糖的水凝胶是一种具有良好生物相容性的出色抗菌伤口敷料候选材料。
