Dong Haoran, Chen Hui, Xu Bing, Tan Yingru, Ling Qun, Shi Liang
Key Laboratory of Microbiological of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Life (Basel). 2022 Dec 26;13(1):68. doi: 10.3390/life13010068.
is a valuable medicinal fungus. is rich in a variety of chemical compounds with medicinal value, among which are flavonoids. Therefore, increasing the content of flavonoids in is beneficial for its potential use in medicinal applications. This study demonstrated that exogenous treatment with citric acid (CA) could significantly increase flavonoid accumulation in . Additionally, we found that CA induced the biosynthesis of flavonoids in a concentration- and time-dependent manner. The flavonoid content could be increased up to 60.96 mg/g when using the treatment with 2.77 mM citric acid for 69.74 h, which was determined by using the response surface method. The changes in the fingerprint profiles of flavonoids with the treatment of CA as an exogenous inducer were also analyzed. In this study, the effect of citric acid as the exogenous inducer on the flavonoid content of was studied, and the processing conditions were optimized through the surface response curve. This approach provides novel insights and a theoretical basis for the production of high-quality .
是一种珍贵的药用真菌。富含多种具有药用价值的化合物，其中包括黄酮类化合物。因此，提高中黄酮类化合物的含量有利于其在药用领域的潜在应用。本研究表明，用柠檬酸（CA）进行外源处理可显著增加中黄酮类化合物的积累。此外，我们发现CA以浓度和时间依赖性方式诱导黄酮类化合物的生物合成。使用2.77 mM柠檬酸处理69.74 h时，黄酮类化合物含量可提高至60.96 mg/g，这是通过响应面法测定的。还分析了以CA作为外源诱导剂处理后中黄酮类化合物指纹图谱的变化。在本研究中，研究了柠檬酸作为外源诱导剂对中黄酮类化合物含量的影响，并通过表面响应曲线优化了处理条件。该方法为高品质的生产提供了新的见解和理论依据。
