Department of Dermatology, Cosmetology and Aesthetic Surgery, Medical College, Jan Kochanowski University, IX Wieków Kielc 19, 35-317 Kielce, Poland.
Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Kielnarowa 386a, 36-020 Tyczyn, Poland.
Molecules. 2023 Jan 15;28(2):868. doi: 10.3390/molecules28020868.
In this study, methanol and water-methanol extracts of borage () herb dried using various methods were analysed for their phenolic profile and biological activity. Twelve compounds, including flavonoids (astragalin, kaempferol 4-glucoside, rutoside, and vitexin) and phenolic acids (caffeic, chlorogenic, 3,4-dihydroxyphenylacetic, ferulic, -hydroxybenzoic, protocatechuic, rosmarinic, and syringic), were determined qualitatively and quantitatively in extracts by the HPLC-DAD method. The highest total flavonoid content was confirmed for the methanol extract from the hot-air-dried herb, while the methanol extract from the air-dried herb was most abundant in phenolic acids. The results of in vitro tests on human keratinocytes (HaCaT) and fibroblasts (BJ) showed that the extracts were able to reduce the intracellular level of reactive oxygen species in skin cells. Tests performed to assess inhibition of protein denaturation, lipoxygenase activity, and proteinase activity demonstrated that borage extracts have anti-inflammatory properties. In addition, the methanol extract of the herb dried in a convection oven showed the strongest inhibition of both collagenase and elastase activity, which is indicative of anti-ageing properties. The results show that the borage extracts are a source of valuable bioactive compounds with beneficial properties in the context of skin cell protection.
在这项研究中，分析了用不同方法干燥的琉璃苣（Borago officinalis）草的甲醇和水-甲醇提取物的酚类成分和生物活性。通过 HPLC-DAD 方法，在 提取物中定性和定量确定了 12 种化合物，包括类黄酮（芦丁、山柰酚 4-葡萄糖苷、芦丁苷和牡荆素）和酚酸（咖啡酸、绿原酸、3,4-二羟基苯乙酸、阿魏酸、-羟基苯甲酸、原儿茶酸、迷迭香酸和丁香酸）。结果表明，热空气干燥草的甲醇提取物总黄酮含量最高，而空气干燥草的甲醇提取物中酚酸含量最丰富。体外试验结果表明，这些提取物能够降低皮肤细胞内活性氧的水平。对蛋白质变性、脂氧合酶活性和蛋白酶活性抑制的测试表明，琉璃苣提取物具有抗炎作用。此外，在对流烤箱中干燥的草的甲醇提取物对胶原蛋白酶和弹性蛋白酶活性的抑制作用最强，这表明其具有抗衰老特性。结果表明，琉璃苣提取物是具有有益特性的有价值生物活性化合物的来源，可用于保护皮肤细胞。
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