Nowak Anna, Zagórska-Dziok Martyna, Perużyńska Magdalena, Cybulska Krystyna, Kucharska Edyta, Ossowicz-Rupniewska Paula, Piotrowska Katarzyna, Duchnik Wiktoria, Kucharski Łukasz, Sulikowski Tadeusz, Droździk Marek, Klimowicz Adam
Department of Cosmetic and Pharmaceutical Chemistry, Pomeranian Medical University in Szczecin, Szczecin, Poland.
Department of Technology of Cosmetic and Pharmaceutical Products, Medical College, University of Information Technology and Management in Rzeszow, Rzeszów, Poland.
Front Pharmacol. 2022 Jul 1;13:896706. doi: 10.3389/fphar.2022.896706. eCollection 2022.
L. is an ethnomedicinal plant known as a medicinal plant in many regions of the world, among others, in various skin diseases. Despite the great interest in this plant, there are still few reports of biological activity of ready-made dermatological or cosmetical preparations containing the extracts. The antioxidant, anti-ageing, anti-inflammatory, antibacterial properties and toxicity, wound healing, and skin permeation of topical hydrogels containing extracts (HEas) was assessed. First, the plant extracts were prepared using three solvents: 70% (v/v) ethanol, 70% (v/v) isopropanol and water, next by preparing hydrogels witch by dry extracts (HEa-EtOH), (HEa-iPrOH) and (HEa-WA), respectively. Finally, the content of selected phenolic acids in the HEas was evaluated by high-performance liquid chromatography (HPLC). All the HEas were characterized by high antioxidant activity. The most increased antibacterial activity was observed for a strain of ATCC 49619, , ATCC 29212, , ATCC 9341 and while the strains of , , and were the least sensitive. All the HEas showed a reduction in the activity of lipoxygenase enzymes, proteases, and inhibition of protein denaturation. The HEa-EtOH and HEa-iPrOH also enhanced the wound healing activity of HDF cells. Additionally, penetration studies were performed using the Franz diffusion cells. These studies showed that the active ingredients contained in penetrate through human skin and accumulate in it. Furthermore, the hydrogels containing extracts showed a broad spectrum of activity. Therefore, they can be considered as an interesting alternative for dermatologic and cosmetic preparations.
L. 是一种民族药用植物，在世界许多地区都被视为药用植物，尤其用于治疗各种皮肤病。尽管人们对这种植物有着浓厚的兴趣，但关于含有其提取物的现成皮肤科或化妆品制剂的生物活性的报道仍然很少。对含有提取物的局部水凝胶（HEas）的抗氧化、抗老化、抗炎、抗菌特性及毒性、伤口愈合和皮肤渗透进行了评估。首先，使用三种溶剂制备植物提取物：70%（v/v）乙醇、70%（v/v）异丙醇和水，接下来分别通过用干提取物制备水凝胶，即（HEa - EtOH）、（HEa - iPrOH）和（HEa - WA）。最后，通过高效液相色谱法（HPLC）评估HEas中选定酚酸的含量。所有的HEas都具有高抗氧化活性。对于ATCC 49619菌株、 、ATCC 29212菌株、 、ATCC 9341菌株和 ，观察到其抗菌活性增加最为明显，而 、 和 菌株最不敏感。所有的HEas都显示出脂氧合酶、蛋白酶活性降低以及蛋白质变性受到抑制。HEa - EtOH和HEa - iPrOH还增强了人皮肤成纤维细胞（HDF细胞）的伤口愈合活性。此外，使用Franz扩散池进行了渗透研究。这些研究表明，提取物中含有的活性成分可穿透人体皮肤并在其中蓄积。此外，含有提取物的水凝胶显示出广泛的活性谱。因此，它们可被视为皮肤科和化妆品制剂的一个有趣替代品。
