Parra Beatriz, Contreras Adolfo, Mina José Herminsul, Valencia Mayra Eliana, Grande-Tovar Carlos David, Valencia Carlos Humberto, Ramírez Cristina, Bolívar Germán Armando
Grupo de Virus Emergentes y Enfermedad (VIREM), Departamento de Microbiología, Facultad de Salud, Universidad del Valle, Calle 4B No. 36-00, Santiago de Cali 760032, Colombia.
Grupo Medicina Periodontal, Escuela de Odontología, Facultad de Salud, Universidad del Valle, Calle 4B No. 36-00, Santiago de Cali 760043, Colombia.
Nanomaterials (Basel). 2023 Jan 14;13(2):343. doi: 10.3390/nano13020343.
Previous studies have suggested that graphene oxide (GO) has some antiviral capacity against some enveloped viruses, including SARS-CoV-2. Given this background, we wanted to test the in vitro antiviral ability to GO using the viral plaque assay technique. Two-dimensional graphene oxide (GO) nanoparticles were synthesized using the modified Hummers method, varying the oxidation conditions to achieve nanoparticles between 390 and 718 nm. The antiviral activity of GO was evaluated by experimental infection and plaque formation units assay of the SARS-CoV-2 virus in VERO cells using a titrated viral clinical isolate. It was found that GO at concentrations of 400 µg/mL, 100 µg/mL, 40 µg/mL, and 4 µg/mL was not toxic to cell culture and also did not inhibit the infection of VERO cells by SARS-CoV-2. However, it was evident that GO generated a novel virus entrapment phenomenon directly proportional to its concentration in the suspension. Similarly, this effect of GO was maintained in assays performed with the Zika virus. A new application for GO nanoparticles is proposed as part of a system to trap viruses in surgical mask filters, air conditioning equipment filters, and air purifier filters, complemented with the use of viricidal agents that can destroy the trapped viruses, an application of broad interest for human beings.
先前的研究表明，氧化石墨烯（GO）对包括SARS-CoV-2在内的一些包膜病毒具有一定的抗病毒能力。在此背景下，我们想使用病毒蚀斑测定技术测试GO的体外抗病毒能力。采用改良的Hummers方法合成二维氧化石墨烯（GO）纳米颗粒，改变氧化条件以获得390至718纳米之间的纳米颗粒。使用滴定的病毒临床分离株，通过在VERO细胞中对SARS-CoV-2病毒进行实验感染和蚀斑形成单位测定，评估GO的抗病毒活性。结果发现，浓度为400 µg/mL、100 µg/mL、40 µg/mL和4 µg/mL的GO对细胞培养无毒，也不抑制SARS-CoV-2对VERO细胞的感染。然而，很明显，GO产生了一种新的病毒捕获现象，该现象与其在悬浮液中的浓度成正比。同样，在对寨卡病毒进行的测定中，GO的这种作用也得以维持。作为一种在手术口罩过滤器、空调设备过滤器和空气净化器过滤器中捕获病毒的系统的一部分，提出了GO纳米颗粒的一种新应用，并辅以使用能够破坏捕获病毒的杀病毒剂，这是一项对人类具有广泛意义的应用。
