Fabricky Mihai M C, Gabor Alin-Gabriel, Milutinovici Raluca Adriana, Watz Claudia Geanina, Avram Ștefana, Drăghici George, Mihali Ciprian V, Moacă Elena-Alina, Dehelean Cristina Adriana, Galuscan Atena, Buzatu Roxana, Duma Virgil-Florin, Negrutiu Meda-Lavinia, Sinescu Cosmin
Department of Prosthodontics, Faculty of Dental Medicine, Victor Babeș University of Medicine and Pharmacy, 9 Revolutiei 1989 Ave., 300070 Timisoara, Romania.
Department of Prostheses Technology and Dental Materials, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy of Timisoara, 9 Revolutiei 1989 Ave., 300070 Timisoara, Romania.
Materials (Basel). 2021 Apr 27;14(9):2266. doi: 10.3390/ma14092266.
The design and development of ceramic structures based on 3D scaffolding as dental bone substitutes has become a topic of great interest in the regenerative dentistry research area. In this regard, the present study focuses on the development of two scaffold-type structures obtained from different commercial dental ceramics by employing the foam replication method. At the same time, the study underlines the physicochemical features and the biological profiles of the newly developed scaffolds, compared to two traditional Cerabone materials used for bone augmentation, by employing both the in vitro Alamar blue proliferation test at 24, 48 and 96 h poststimulation and the in ovo chick chorioallantoic membrane (CAM) assay. The data reveal that the newly developed scaffolds express comparable results with the traditional Cerabone augmentation masses. In terms of network porosity, the scaffolds show higher pore interconnectivity compared to Cerabone granules, whereas regarding the biosafety profile, all ceramic samples manifest good biocompatibility on primary human gingival fibroblasts (HGFs); however only the Cerabone samples induced proliferation of HGF cells following exposure to concentrations of 5 and 10 µg/mL. Additionally, none of the test samples induce irritative activity on the vascular developing plexus. Thus, based on the current results, the preliminary biosecurity profile of ceramic scaffolds supports the usefulness for further testing of high relevance for their possible clinical dental applications.
基于三维支架的陶瓷结构作为牙骨替代物的设计与开发，已成为再生牙科研究领域备受关注的课题。在此方面，本研究聚焦于通过泡沫复制法从不同商用牙科陶瓷获得的两种支架型结构的开发。同时，与两种用于骨增量的传统Cerabone材料相比，本研究通过在刺激后24、48和96小时进行体外阿拉玛蓝增殖试验以及鸡胚绒毛尿囊膜（CAM）试验，突出了新开发支架的物理化学特征和生物学特性。数据显示，新开发的支架与传统Cerabone增量材料表现出可比的结果。在网络孔隙率方面，支架与Cerabone颗粒相比显示出更高的孔隙连通性，而在生物安全性方面，所有陶瓷样品在原代人牙龈成纤维细胞（HGFs）上均表现出良好的生物相容性；然而，只有Cerabone样品在暴露于5和10μg/mL浓度时诱导了HGF细胞的增殖。此外，没有一个测试样品对血管发育丛诱导刺激性活性。因此，基于当前结果，陶瓷支架的初步生物安全性特征支持其在牙科临床应用中进行进一步高相关性测试的实用性。
