Andrade-Pinheiro Jacqueline Cosmo, Sobral de Souza Celestina Elba, Ribeiro Daiany Alves, Silva Andressa de Alencar, da Silva Viviane Bezerra, Dos Santos Antonia Thassya Lucas, Juno Alencar Fonseca Victor, de Macêdo Delmacia Gonçalves, da Cruz Rafael Pereira, Almeida-Bezerra José Weverton, Machado Antonio Júdson Targino, de Freitas Thiago Sampaio, de Brito Edy Sousa, Ribeiro Paulo Riceli Vasconcelos, da Costa José Galberto Martins, Coutinho Henrique Douglas Melo, Kowalska Grażyna, Rowiński Rafał, Kowalski Radosław, Morais-Braga Maria Flaviana Bezerra
Pimenta Campus, Regional University of Cariri (URCA), Av. Cel Antônio Luis, 1161, Pimenta, Crato 63105-010, Brazil.
Laboratório de Bioensaios, Federal University of Cariri (UFCA), R. Olegário Emidio de Araujo, s/n, Centro, Brejo Santo 63260-000, Brazil.
Plants (Basel). 2023 Jan 16;12(2):415. doi: 10.3390/plants12020415.
Fungi of the genus are responsible for invasive candidiasis, which affects people all over the world and has high mortality rates. This is due to their virulence factors, which give them great resistance and pathogenicity. In addition, the emergence of multidrug-resistant strains makes it difficult to treat these infections. In this way, natural products have emerged as an alternative to standard drugs, where plants known for their medicinal properties such as become attractive to research. The present work aimed to analyze the ethanol extract of leaves against standard strains of , and using broth microdilution techniques. The identification of the compounds in leaves by LC-MS revealed the presence of a wide variety of substances such as carboxylic acids and terpenes, with flavonoids and fatty acids being more evident. The antifungal assays showed that the extract was not able to inhibit the growth of the tested strains at concentrations with a clinical relevance. However, at higher concentrations, it was able to inhibit the fungal dimorphism of and . It is possible that the extract has potential as an inhibitor of fungal virulence factors without affecting the cell viability. Further research should be carried out in order to assess its inhibitory potential for other fungal virulence factors.
该属真菌可引发侵袭性念珠菌病，这种疾病在全球范围内影响着人们，且死亡率很高。这归因于它们的毒力因子，这些因子赋予了它们很强的抵抗力和致病性。此外，多重耐药菌株的出现使得治疗这些感染变得困难。如此一来，天然产物已成为标准药物的一种替代选择，像那些以药用特性闻名的植物就变得吸引研究人员。本研究旨在采用肉汤微量稀释技术，分析[植物名称]叶的乙醇提取物对[真菌名称1]、[真菌名称2]和[真菌名称3]的标准菌株的作用。通过液相色谱 - 质谱联用技术对[植物名称]叶中的化合物进行鉴定，结果显示存在多种物质，如羧酸和萜类化合物，其中黄酮类化合物和脂肪酸更为明显。抗真菌试验表明，在具有临床相关性的浓度下，该提取物无法抑制受试菌株的生长。然而，在较高浓度下，它能够抑制[真菌名称1]和[真菌名称2]的真菌二态性。[植物名称]提取物有可能作为一种不影响细胞活力的真菌毒力因子抑制剂。为了评估其对其他真菌毒力因子的抑制潜力，应开展进一步的研究。
