Guo Yongyao, Wang Yue, Yin Chunyan, Wang Jianhui, Chen Ye
College of Fisheries, Zhejiang Ocean University, Zhoushan, China.
College of Fisheries, Zhejiang Marine Fisheries Research Institute, Zhoushan, China.
Mitochondrial DNA B Resour. 2023 Jan 15;8(1):133-135. doi: 10.1080/23802359.2022.2163598. eCollection 2023.
The differentiation between and species based only on morphology is ambiguous; however, phylogenetic analysis using their mitogenome sequences provides conclusive results. Here, the phylogenetic position of (Herre, 1940) was determined using its mitogenome data. Total DNA from was sequenced using the Illumina NovaSeq6000 platform, and annotation of mitochondrial genes was performed using MITOS2. Phylogenetic trees were constructed using the complete mitogenomes of 16 fish species. The mitogenome of was found to be 16584 bp long, containing two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and three non-coding control regions. The genome showed a slight A + T bias (A + = 56.46%). Most PCGs were found to be located on the L-strand. Results of the phylogenetic analysis showed that is closely related to . Our results will help to clarify the phylogenetic relationship between and species.
仅基于形态学对[物种名称1]和[物种名称2]进行区分是不明确的；然而，使用它们的线粒体基因组序列进行系统发育分析可提供确凿的结果。在此，利用[物种名称1]（Herre，1940）的线粒体基因组数据确定了其系统发育位置。使用Illumina NovaSeq6000平台对[物种名称1]的总DNA进行测序，并使用MITOS2对线粒体基因进行注释。利用16种鱼类的完整线粒体基因组构建了系统发育树。发现[物种名称1]的线粒体基因组长度为16584 bp，包含两个核糖体RNA基因、13个蛋白质编码基因、22个转运RNA基因和三个非编码控制区。该基因组显示出轻微的A+T偏好（A+T = 56.46%）。发现大多数蛋白质编码基因位于轻链上。系统发育分析结果表明，[物种名称1]与[物种名称2]密切相关。我们的结果将有助于阐明[物种名称1]和[物种名称2]之间的系统发育关系。
