湖羊中[具体基因名称缺失]基因的多态性及其与饲料转化率的关联

Polymorphisms in and genes and their association with feed conversion ratio in Hu sheep.

作者信息

Lin Changchun, Wang Weimin, Zhang Deyin, Huang Kai, Li Xiaolong, Zhang Yukun, Zhao Yuan, Wang Jianghui, Zhou Bubo, Cheng Jiangbo, Xu Dan, Li Wenxin, Zhao Liming, Ma Zongwu, Yang Xiaobin, Huang Yongliang, Cui Panpan, Liu Jia, Zeng Xiwen, Zhai Rui, Sun Landi, Weng Xiuxiu, Wu Weiwei, Zhang Xiaoxue, Zheng Wenxin

机构信息

College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.

The State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.

出版信息

Front Vet Sci. 2023 Jan 5;9:1010045. doi: 10.3389/fvets.2022.1010045. eCollection 2022.

DOI:10.3389/fvets.2022.1010045

PMID:36686193

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9850526/

Abstract

In animal husbandry, feed efficiency is a crucial economic trait. In this study, the general linear model was used to perform association analysis for various genotypes and feed conversion ratio (FCR)-related traits. Reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression of and mRNA levels in 10 tissues from 6 sheep. The results showed that SNPs in the NC_040257.1:c.625 T > C and NC_040261.1:g.9905 T > C were analyzed whether they were associated to feed efficiency parameters in Hu sheep (body weight, feed intake, average daily growth, and feed conversion ratio). NC_040257.1:c.625 T > C was shown to be significantly associated with body weight at 80, 100, and 120 days as well as feed conversion ratio ( < 0.05), whereas NC_040261.1:g.9905 T > C was found to be significantly associated with average daily weight gain from 80-140 days (ADG80-140) and FCR ( < 0.05). In Hu sheep, the CC genotypes of SHISA3 and RFC3 were the most common genotypes related to feed efficiency traits. Furthermore, the feed conversion ratio of the combined genotypes TT -CC , TT -CT , TT -TT , CT -CC and CT -CT was significantly better than the FCR of CC -TT . RT-qPCR results showed that the expression levels of were lower in the lung than in spleen, kidney, muscle and lymph ( < 0.05), and RFC3 was the lung had a highly significant higher expression level than the heart, liver, spleen, and muscle ( < 0.01). In conclusion, and polymorphisms can be used as genetic markers for improving feed conversion efficiency in Hu sheep.

摘要

在畜牧业中，饲料效率是一个关键的经济性状。在本研究中，使用一般线性模型对各种基因型与饲料转化率（FCR）相关性状进行关联分析。采用逆转录定量PCR（RT-qPCR）检测6只绵羊10个组织中SHISA3和RFC3 mRNA水平的表达。结果表明，分析了NC_040257.1:c.625 T>C和NC_040261.1:g.9905 T>C中的单核苷酸多态性（SNPs）是否与湖羊的饲料效率参数（体重、采食量、平均日增重和饲料转化率）相关。结果显示，NC_040257.1:c.625 T>C与80、100和120日龄的体重以及饲料转化率显著相关（P<0.05），而NC_040261.1:g.9905 T>C与80 - 140天的平均日增重（ADG80 - 140）和FCR显著相关（P<0.05）。在湖羊中，SHISA3和RFC3的CC基因型是与饲料效率性状相关的最常见基因型。此外，组合基因型TT - CC、TT - CT、TT - TT、CT - CC和CT - CT的饲料转化率显著优于CC - TT的FCR。RT-qPCR结果显示，SHISA3在肺中的表达水平低于脾脏、肾脏、肌肉和淋巴结（P<0.05），而RFC3在肺中的表达水平显著高于心脏、肝脏、脾脏和肌肉（P<0.01）。总之，SHISA3和RFC3多态性可作为提高湖羊饲料转化效率的遗传标记。