Du Kun, Zeng Meng-Nan, Wang Yan-Zhi, Liu Yu-Fei, Feng Wei-Sheng
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Construction By Henan Province & Education Ministry of People's Republic of China, Zhengzhou, 450046, China.
J Nat Med. 2023 Mar;77(2):397-402. doi: 10.1007/s11418-023-01679-w. Epub 2023 Jan 23.
Two new β-carboline alkaloids, anemonilins A and B (1-2), and two known β-carboline alkaloids, flazine (3) and 4-(9H-β-carbolin-l-yl)-4-oxo-butyric acid (4), were isolated from the roots of Anemone altaica. The structures of the isolated compounds were elucidated with spectroscopic and spectrometric methods (1D and 2DNMR, HRESIMS). Compounds 2 and 4 significantly attenuated the growth inhibition induced by lipopolysaccharide (LPS) in normal rat kidney tubule epithelioid (NRK52e) cells (p < 0.05 or p < 0.01). Furthermore, compound 2 significantly reduced the apoptosis (p < 0.05) and the caspase-3/9 expression of NRK52e cells induced by LPS.
从阿尔泰银莲花的根部分离出两种新的β-咔啉生物碱，即银莲花宁A和B（1-2），以及两种已知的β-咔啉生物碱，即黄酮嗪（3）和4-（9H-β-咔啉-1-基）-4-氧代丁酸（4）。通过光谱和光谱法（一维和二维核磁共振、高分辨电喷雾电离质谱）阐明了分离出的化合物的结构。化合物2和4显著减轻了脂多糖（LPS）诱导的正常大鼠肾小管上皮样（NRK52e）细胞的生长抑制（p<0.05或p<0.01）。此外，化合物2显著降低了LPS诱导的NRK52e细胞的凋亡（p<0.05）和半胱天冬酶-3/9的表达。
