Laboratory of Phytopathology, Institute of Pesticides and Environmental Protection, 11080, Belgrade, Serbia.
Sunflower Department, Institute of Field and Vegetable Crops, 21000, Novi Sad, Serbia.
Sci Rep. 2023 Jan 25;13(1):1433. doi: 10.1038/s41598-023-28562-8.
Rubbery taproot disease (RTD) of sugar beet was recently associated with the plant pathogenic bacterium 'Candidatus Phytoplasma solani' (CaPsol) and reported throughout the Pannonian Plain with variations in severity. Tracing CaPsol epidemiological pathways was performed in the experimental sugar beet field in Rimski Šančevi (Serbia) in 2020-2021, where an RTD outbreak was recently recorded. A molecular epidemiology approach was applied to the study of three RTD occurrence scenarios: epidemic, non-epidemic and 'absence of RTD'. As a result, Hyalesthes obsoletus ex Convolvulus arvensis was detected as a CaPsol vector to sugar beet, while two other cixiids were identified for the first time as vectors of the CaPsol-induced plant disease in crops: Reptalus quinquecostatus and R. cuspidatus. R. quinquecostatus was proposed culpable for the 2020 RTD epidemic outbreak in Rimski Šančevi when dSTOLg CaPsol strain predominated in the RTD-affected sugar beet, whereas R. cuspidatus had a negligible role in RTD occurrence and displayed ambiguous involvement in CaPsol epidemiology on a wider scale. The temporal discrepancy of the offset of CaPsol dissemination and disease occurrence is the main obstacle in predicting CaPsol-induced diseases. Predicting disease occurrence and severity can only be achieved by gaining a better understanding of CaPsol epidemiological pathways and insect vectors involved in disease outbreaks.
甜菜根肿病（RTD）最近与植物病原细菌“韧皮部杆菌‘（CaPsol）有关，并在整个潘诺尼亚平原报道，严重程度不同。2020-2021 年在里姆斯基·桑切维（塞尔维亚）的实验甜菜田追踪 CaPsol 流行病学途径，最近记录到 RTD 爆发。应用分子流行病学方法研究了三种 RTD 发生情况：流行、非流行和“无 RTD”。结果表明，野菟丝子是甜菜的 CaPsol 传播媒介，而另外两种木虱首次被确定为作物中 CaPsol 诱导植物病害的传播媒介：五刺花腿盲蝽和尖胸花腿盲蝽。2020 年里姆斯基·桑切维的 RTD 流行爆发时，dSTOLg CaPsol 菌株在受 RTD 影响的甜菜中占主导地位，R. quinquecostatus 被认为是罪魁祸首，而 R. cuspidatus 在 RTD 发生中的作用微不足道，在更大范围内对 CaPsol 流行病学的参与也模棱两可。CaPsol 传播和疾病发生的时间差异是预测 CaPsol 诱导疾病的主要障碍。只有更好地了解参与疾病爆发的 CaPsol 流行病学途径和昆虫媒介，才能预测疾病的发生和严重程度。
