Therhaag Eva, Schneider Bernd, Zikeli Kerstin, Maixner Michael, Gross Jürgen
Institute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, 69221 Dossenheim, Germany.
Institute for Plant Protection in Fruit Crops and Viticulture, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, 76833 Siebeldingen, Germany.
Insects. 2024 Mar 13;15(3):189. doi: 10.3390/insects15030189.
In Germany, the phloem-sucking planthopper (Hemiptera: Cixiidae) currently represents the epidemiological driver for the spread of the syndrome "Basses Richesses" in sugar beets, which results in a reduced sugar content and an economic loss for the farmers. This disease is associated with the γ-proteobacterium ' Arsenophonus phytopathogenicus' and the Stolbur phytoplasma ' Phytoplasma solani'. Recently, was found in potato fields in Germany and is associated with Stolbur-like symptoms in this crop. In this study, we confirmed that the vector completes its lifecycle on sugar beets as well as on potatoes when reared under controlled conditions. Transmission experiments with adults of this vector combined with molecular analyses showed, for the first time, that both pathogens are transmitted by this vector to potatoes. For an accurate assessment of the Ca. P. solani and Ca. A. phytopathogenicus titers in the vector and host plants, gBlocks derived from the 20- and 16S rRNA genes were employed, respectively. For Ca. P. solani, the limit of detection was determined in potato and sugar beet material. The results of this study will further the research on the epidemiology of the syndrome "Basses Richesses" and "Stolbur" diseases and the response of host plants and vector insects to both pathogens.
在德国，吸食韧皮部的飞虱（半翅目：蜡蝉科）目前是甜菜中“Basses Richesses”综合征传播的流行病学驱动因素，该综合征会导致糖分含量降低，给农民造成经济损失。这种病害与γ-变形菌“植物致病嗜砷菌”和“马铃薯 stolbur 植原体”有关。最近，在德国的马铃薯田中发现了它，并与这种作物上的类 stolbur 症状有关。在本研究中，我们证实，在受控条件下饲养时，该传毒介体在甜菜和马铃薯上均能完成其生命周期。用这种传毒介体成虫进行的传播实验结合分子分析首次表明，这两种病原体均能由该传毒介体传播至马铃薯。为了准确评估该传毒介体和寄主植物中马铃薯 stolbur 植原体和植物致病嗜砷菌的滴度，分别采用了源自 23S 和 16S rRNA 基因的 gBlocks。对于马铃薯 stolbur 植原体，在马铃薯和甜菜材料中确定了检测限。本研究结果将推动对“Basses Richesses”综合征和“Stolbur”病害流行病学以及寄主植物和传毒介体昆虫对这两种病原体反应的研究。
