Yao Zhangliang, Miao Jiashun, Wang Baojun, Xu Weidong, Wang Yeqing, Lu Qiang, Zhang Jidong
Institute of Eco-Environmental Sciences, Jiaxing Academy of Agricultural Sciences, Jiaxing, China.
Xianghu Laboratory, Hangzhou, China.
Front Plant Sci. 2024 Oct 11;15:1428943. doi: 10.3389/fpls.2024.1428943. eCollection 2024.
Continuous cropping in greenhouse cultivation often leads to increased pest and disease problems, reducing crop quality and yield. Crop rotation is a common strategy to address these issues. This study compared the growth of Chinese cabbage ( var. ) following rotations with ginger () and sponge gourd (). The Chinese cabbage exhibited normal growth following ginger rotation but showed abnormal growth after sponge gourd rotation. The study investigated the underlying causes by analyzing soil physicochemical properties and rhizosphere microbial communities of Chinese cabbage using 16S rRNA and ITS sequencing. The results revealed that soil from ginger-Chinese cabbage rotation had higher levels of soil organic carbon (SOC) and available phosphorus (AP), but lower total nitrogen (TN) and available potassium (AK). Despite similar alpha-diversity for both bacterial and fungal communities, distinct bacterial and fungal community structures between two rotation cropping systems were observed. This suggests that even if the alpha-diversity does not change, the composition of the microbial community can shift in ways that might influence soil health and plant growth. Furthermore, redundancy analysis revealed a significant correlation between microbial community structures and soil physicochemical properties of two rotation cropping systems. The SOC and TN were revealed to be the most significant of the investigated soil physicochemical parameters with respect to the variation of both bacterial and fungal assemblages, respectively. The identified biomarkers in bacterial community composition further emphasize the potential for specific microbes to influence crop health positively or negatively. We found that the indicator genera of the bacterial community composition of the ginger-Chinese cabbage rotation system were (genus), (order), (family), and , which are known as producers of secondary metabolites, such as antibiotics. These findings highlight the importance of crop selection in rotation strategies for optimizing agricultural outcomes.
温室栽培中的连作常常导致病虫害问题增加，降低作物品质和产量。轮作是解决这些问题的常用策略。本研究比较了大白菜（品种）在与生姜（）和丝瓜（）轮作后的生长情况。大白菜在与生姜轮作后生长正常，但在与丝瓜轮作后出现异常生长。该研究通过使用16S rRNA和ITS测序分析大白菜的土壤理化性质和根际微生物群落来探究其潜在原因。结果表明，生姜-大白菜轮作的土壤中土壤有机碳（SOC）和有效磷（AP）含量较高，但总氮（TN）和有效钾（AK）含量较低。尽管细菌和真菌群落的α多样性相似，但在两种轮作种植系统之间观察到了不同的细菌和真菌群落结构。这表明，即使α多样性不变，微生物群落的组成也可能以影响土壤健康和植物生长的方式发生变化。此外，冗余分析揭示了两种轮作种植系统的微生物群落结构与土壤理化性质之间存在显著相关性。就细菌和真菌组合的变化而言，SOC和TN分别是所研究的土壤理化参数中最显著的参数。在细菌群落组成中鉴定出的生物标志物进一步强调了特定微生物对作物健康产生积极或消极影响的潜力。我们发现，生姜-大白菜轮作系统细菌群落组成的指示属为（属）、（目）、（科）和，它们是已知的次生代谢产物如抗生素的生产者。这些发现凸显了在轮作策略中选择作物对于优化农业产出的重要性。
